蜈蚣蛋白水解产物ACE抑制活性的初步研究

Primary Study on Hydrolysation of Centipedes Protein and Evaluation of Its ACE Inhibitory Activity

目的:对动物药蜈蚣(Scolopendra subspinipes muitilans L.Koch.)蛋白组分进行测定,并对各组分的酶解物进行血管紧张素转化酶(ACE)体外活性评价。方法:采用顺序抽提法依次进行清蛋白、球蛋白、醇溶蛋白和谷蛋白的提取,用Brandford和凯式定氮法进行蛋白质含量的测定。利用胃蛋白酶水解蜈蚣的4类蛋白及残渣,酶解液超滤冻干得到小分子量多肽(≤3 k D),采用高效液相色谱法对其ACE抑制活性进行测定。结果:蜈蚣总蛋白质含量为(62.69±1.41)%。4大类蛋白及残渣中蛋白占总蛋白含量的百分比分别为:清蛋白(6.42±0.31)%;球蛋白(7.94±0.24)%;醇溶蛋白(4.31±0.34)%;谷蛋白(40.66±0.56)%;残渣(25.78±0.60)%。这4大类蛋白及残渣蛋白酶解物在1 mg·m L-1浓度下的体外ACE抑制率分别为:50.28%、57.37%、31.15%、58.99%、80.81%。结论:蜈蚣中蛋白质含量丰富,且蛋白水解产物均表现出一定的ACE抑制作用,其中残渣蛋白和谷蛋白的ACE抑制作用较强,为蜈蚣临床用药及功能食品的开发提供了理论支持。

This study was aimed to analyze the major protein composition of centipedes（Scolopendra subspinipes mutilans L.Koch.）, and the ACE inhibitory activity of its hydrolysis. Albumins, gulbulins, coixins and glutelins were sequentially extracted from centipedes flour with corresponding buffer and then quantified by Kjeldahl method and Brandford. Hydrolysation of four kinds of proteins of centipedes and the residues were conducted with pepsin. The hydrolysis was ultrafiltrated（MWCO = 3 000） and lyophilized. The peptides（≤ 3 k D） were obtained to evaluate the ACE inhibitory activity by RP-HPLC. The results showed that the total protein content of centipedes was（62.69±1.41）%. Among which the contents of albumins, globulins, coixins, glutelins and residual were accounting for（6.42±0.31）%,（7.94±0.24）%,（4.31±0.34）%,（40.66±0.56）% and（25.78±0.60）%, respectively. The inhibition rate of hydrolysis of four kinds of protein and residual were 50.28%, 57.37%, 31.15%, 58.99%, 80.81%, respectively. It was concluded that centipedes were rich in protein and the hydrolyzate of all proteins manifested ACE inhibitory activity at different extent. The residual and glutelins indicated strong ACE inhibitory potential by hydrolysis. This research provided valuable sights for exploring hypotensive activity and functional food from centipedes.