大豆异黄酮对骨质疏松大鼠种植体骨结合疗效的评价

Curative effect appraising of soybean isoflavones on implant osseointegration in rats with osteoporosis

目的:观察大豆异黄酮对骨质疏松大鼠种植体周围骨以及骨结合强度的影响。方法:选用4月龄雌性SD大鼠40只,用随机区组的方法将其分为假手术组(SHAM,10只)、卵巢切除组(OVX,10只)、卵巢切除+高剂量大豆异黄酮组(OVX+SIH,10只)和卵巢切除+低剂量大豆异黄酮组(OVX+SIL,10只)共4组。术后8周所有实验动物双侧胫骨干骺端分别植入钛螺纹钉1颗,2个药物干预组使用不同剂量SI药物干预12周。所有实验动物取包含螺纹钉的骨组织块进行生物力学、组织形态定量学检查。结果:与OVX组相比,低剂量和高剂量SI均能有效增加种植体的骨结合(组织形态定量学检测),并且增加了种植体的最大脱出力,其中高剂量SI组的种植体稳定性与对照组无显著差别(生物力学检测)。结论:本研究结果说明大豆异黄酮有剂量依赖性地改善骨质疏松大鼠钛螺纹钉的骨结合强度的可能性,增强疏松状态下种植体的稳定性。

Objective： To observe the effect of soybean isoflavones（SI） on bone around implant and integrate strength in rats with osteoporosis. Methods： Female SD rats of 4 months were enrolled and randomly divided into four groups：sham-operated group（SHAM, n=10）, ovariectomized group（OVX,n=10）, ovariectomy＋ high doses of SI group,（OVX＋SIH, n=10）, and ovariectomy＋ low doses of SI group（OVX＋SIL, n=10）. 8 weeks after treatment, titanium implants were inserted into tibia metaphysis bilaterally in each SD rat. Different does of SI were applied for 12 weeks in these two SI-loaded groups. Bone blocks with implants in all these experimental animals were evaluated by biomechanical tests and histomorphometric analysis. Results： Compared with OVX group, histomorphometric analysis indicated that both low and high doses of SI can increase implant osseointegration effectively. The maximal force of implant in biomechanical push-out test was also increased. Biomechanical test results showed there was no significant difference between high doses of SI group and control group in terms of implant stability. Conclusion： These results show that soybean isoflavones can improve implant bone integrate strength in rats with osteoporosis dose-dependently, and facilitate the stability of implant in the osteoporotic bone.