MTA和光固化氢氧化钙及光固化玻璃离子对人牙髓细胞增殖影响的比较

Effects of three endodontic materials on oil proliferation of human dental pulp cells in vitro

目的:通过甲基噻唑基四唑(MTT)法研究三氧化矿物凝聚体(MTA)、光固化氢氧化钙(light-curing calcium hydroxide)及光固化玻璃离子水门汀(Light-cured glass ionomer cement,LGIC)材料对人牙髓细胞(human dental Pulp Cells,HDPCs)增殖的影响,从而为临床应用提供理论依据。方法:配制光固化氢氧化钙、LGIC、MTA三种材料浸提液,按体积分数稀释成不同浓度。用不同浓度的材料浸提液培养,收集培养细胞,用MTT法测定细胞的增殖情况。结果:Ca(OH)2组细胞OD值明显低于其它各组(P<0.05),其它各组之间OD值大小无差别(P>0.05),材料浸提液的浓度对所培养细胞OD值大小无明显影响(P>0.05)。结论:Ca(OH)2对HDPCs的增殖均有较强的抑制作用。LGIC对细胞的增殖无明显促进作用,也无明显抑制作用。MTA对hDPCs增殖无抑制。

Objective： To investigate the effects of calcium hydroxide,mineral trioxide aggregate and light-cured glass ionomer cement on proliferation of hDPCs by MTT tests. Methods： Different concentrations of the eluate of Ca（OH）2,MTA and LGIC were prepared. HDPCs were cultured with the eluate, and cell proliferation was measured by MTT method.Results： Cell proliferation was assessed by MTT, showing that at day 2,4,6,8, OD value of Ca（OH）2 was much lower than other groups. and no significant differentces were founed in MTA, LGIC and DMEM groups. The OD value of hDPCs showed no difference at different time,concentratios and materials. Conclusion： Ca（OH）2 can inhibit the cell proliferation but MTA or LGIC had no negative effects on that. The cytotocity of Ca（OH）2 are stronger than MTA and LGIC. LGIC may have no effect on increasing or decreasing cell proliferation., while MTA has no effect on decreasing cell proliferation.