运动和膳食干预对胰岛素抵抗大鼠氧化应激的影响

Effects of exercise and diet intervention on oxidative stress in insulin resistant rat

目的研究8周的运动和膳食等生活方式干预对胰岛素抵抗大鼠氧化应激的影响。方法采用高脂饲料建立肥胖诱导的胰岛素抵抗大鼠模型,以体重和脂体比作为肥胖程度的衡量标准,以口服糖耐量试验葡萄糖曲线下面积作为糖耐量衡量标准。再分别进行8周的中等强度跑台运动、相对低脂的基础饲料及两者联合干预,以铜试剂法测定血清游离脂肪酸(FFA)含量,以黄嘌呤氧化酶法和硫代巴比妥酸法测定肝脏、脂肪组织和比目鱼肌中超氧化物歧化酶(SOD)活性与丙二醛(MDA)含量,以FFA含量和SOD/MDA衡量大鼠氧化应激状态。结果 8周干预后,3种干预均降低了大鼠体重(P<0.01)、脂体比(P<0.01)和葡萄糖曲线下面积(P<0.01)。膳食和联合干预降低了血清FFA浓度(P<0.01),单独的运动干预没有这种效应。3种干预提高了肝脏和脂肪组织中SOD/MDA(P<0.01),运动和联合干预提高了比目鱼肌中的SOD/MDA(P<0.05),而单独的膳食干预没有这种效应。结论运动和膳食干预能改善大鼠的胰岛素抵抗状态,这与氧化应激状态的缓解有关。

Objective To investigate the effects of exercise, diet and their combination intervention on oxidative stress of insulin resistance rat. Methods Establish obesity-induced insulin resistance rat models. Obesity was assessed by the body weight and lipid ratio. Glucose tolerance was assessed by the integrated area under the curve for glucose （AUCg） during an oral glucose tolerance test （OGTT） , then 8 weeks of exercise, diet, and combination interventions, respectively. To analyze serum free fatty acids （FFA） content, superoxide dismutase （SOD） and maleic dialdehyde （MDA） content in liver, adipose tissue and soleus muscle by biochemical method. Judge oxidative stress by FFA content and SOD/MDA. Results Three kinds of intervention reduced the body weight（P 〈 0.01）, lipid ratio （P 〈 0.01） and AUCg （P 〈 0.01）. Dietary and combination intervention lowered serum free fatty acid concentration（P 〈 0.01 ） , separate exercise intervention had not such effect. Three kinds of intervention increased SOD/MDA the liver and a soleus muscle etary interventi stress Ldipose tissue, ex , dietary interven on may improve ercise and combination intervention improved SOD/MDA Ltion alone had not the effect. Conclusion Exercise and the overall insulin resistance by alleviating oxidative