rs855791和rs3811647多态性对8-14岁人群血清铁蛋白与可溶性转铁蛋白受体水平的影响

Effects of the SNPs on rs855791 and rs3811647 on the levels of SF and sTfR in the group of 8-14

目的探索TMPRSS6基因中rs855791和TF基因中rs3811647的多态性对青少年人群血清铁蛋白(SF)、可溶性转铁蛋白受体(sTfR)水平的影响。方法抽取50名研究对象静脉血并进行DNA提取,经Sequenom Mass Array系统对样本的2个目标位点进行多态性检测。应用t检验和卡方检验分别对2个多态性位点中不同等位基因携带者之间的SF、sTfR的水平进行比较,推测每个位点的多态性对SF、sTfR水平的影响。结果 rs855791位点中A等位基因携带者SF水平(54.0±28.2 ng/ml)较GG纯合子携带者SF水平(33.1±20.2 ng/ml)高(P<0.05),未观察到不同等位基因携带者之间sTfR水平存在差异;rs3811647位点中未观察到不同等位基因携带者之间SF、sTfR水平存在差异。结论 TMPRSS6基因中rs855791位点的多态性对SF的水平可能存在影响,并且其影响作用可能高于TF基因中rs3811647位点的多态性。

Objective To investigate effects on the levels of SF and sTfR by the SNPs of rs855791on TMPRSS6 and rs3811647 on TF in adolescent. Methods DNA was extracted from venous blood which were drawn from 50 subjects, and then the two SNPs of each sample were identified by Sequenom MassArray. T test and chi-square test were selected to identify the relationship between the levels of SF and sTfR in each allele carriers, and then the effects of each SNP on the levels of SF and sTfR would be assessed. Results The level of SF of A allele carriers on rs855791 （54. 0 ± 28.2 ng/ml） was higher than GG carriers （33.1±20.2 ng/ml） （P 〈0.05） , and the discrimination of the level of sTfR between each allele carrier was not observed. The discriminations of the different levels of SF and sTfR among each SNP on rs3811647 were not observed. Conclusions The level of SF may be affected by the SNP of rs855791on TMPRSS6, and the effect of rs3811647 on TF may be weakened by the former one.