胡桃醌对宫颈鳞癌Caski细胞增殖及凋亡作用的研究

Proliferation inhibition and apoptosis induction of Juglone on human cervical cancer Caski cells

目的探讨胡桃醌对宫颈鳞癌Caski细胞增殖及其促凋亡作用的影响,并对其促凋亡的机制进行初步探究。方法选取处于对数生长期的Caski细胞将其分为空白对照组和不同剂量(20、40、60、80和100 mol/L)胡桃醌组,共6组。采用MTT法观察胡桃醌对Caski细胞的抑制作用,并计算出半数抑制浓度(IC50=42.4μmol/L),依据IC50确定胡桃醌的有效浓度;电镜观察40μmol/L胡桃醌对Caski细胞凋亡的形态学特征,Western blot检测凋亡相关蛋白Bcl-2及Bax的表达。结果 MTT试验显示,与对照组比较,除20μmol/L的胡桃醌外,其他各浓度组差异均有统计学意义(P<0.05,P<0.01);电镜下可见凋亡细胞及凋亡小体,Western blot检测显示与正常对照组相比,40μmol/L胡桃醌Bcl-2表达明显降低而Bax表达明显增加(P<0.05)。结论胡桃醌可以抑制Caski的增殖,并诱导细胞凋亡。

Objective To explore the effects of Juglone on proliferation and apoptosis of human cervical cancer Caski cells, and to further study the related mechanism of cell apoptosis. Methods Cultured Caski cells were incubated with 20,40,60,80 and 100 μmol/L juglone for 24 h. The proliferation of Caski cells was detected by methyl thiazolyl tetrazolium （MTT）assay. The cell apoptosis were detected by transmission electron microscope. The expression of Bcl-2 and Bax were detected by Western blot. Results MTT results showed that in different doses of juglone groups, the Caski cell growth was greatly inhibited （P 〈 0.05, P 〈 0.01 ） and showed dose dependent when compared with control group except 20 μ mol/L. The IC50 of juglone was 42.4 p, mol/L. After treatment on Caski cells with 40μmol/L juglone , typicalapoptosis characteristics was observed by transmission electronmicro scope. The expression of Bcl-2 was decreased while the expression of Bax was increased significantly when compared with control group （ P 〈 0.05）. Conclusion Juglone significantly inhibits the proliferation and induces the apoptosis of Caski cells in vitro.