期刊文献+

建立一种HBV突变检测的蝎子实时荧光PCR新方法

Establishment of a new Scorpion real-time fluorescence PCR method to detect the mutation of HBV
下载PDF
导出
摘要 目的为HBV突变检测建立一种有效、快速、简捷的蝎子实时荧光PCR新方法。方法同时用蝎子实时荧光PCR新方法和Sanger测序法对比:对157例HBV患者的血清的DNA进行HBV基因rtN236T突变检测,统计其符合率;通过对混有不同比例的HBV基因rtN236T突变核酸的样本进行对照检测来比较两者的灵敏度。结果在157例HBV患者的血清样本中,蝎子实时荧光PCR新方法检测到rtN236T突变的有69例,而Sanger测序法检测的有68例,其符合率为98.6%;蝎子实时荧光PCR新方法能检测出混有5%突变的样本,其灵敏度达5%,比Sanger测序法高。结论蝎子实时荧光PCR新方法能有效检测到HBV突变,且比Sanger测序法更为简单、快速、灵敏度高,适合临床应用。 Objective To establish an effective, rapid and simple Scorpion real-time PCR method to detect the mutation of HBV. Methods The rtN236T mutation of HBV was detected by Scorpion real-time PCR and Sanger sequencing method, respectively. 157 patients were tested and coincidence rate was calculated. The samples which mixed with different proportions of HBV mutant DNA were tested to compare the sensitivity of Scorpion real-time PCR method and Sanger sequencing method. Results In the DNA of 157 HBV patients, 69 cases with the rtN236 mutation of HBV were detected by Scorpion real-time PCR method while 68 cases were detected by Sanger sequencing method. The coincidence rate of the two methods was 98.6%. The Scorpion real-time PCR method can detect the samples which mixed with 5%HBV mutant DNA , and its sensitivity was 5%, which was higher than that of Sanger sequencing method. Conclusion The mutation of HBV can be detected by the Scorpion real-time PCR. It is more simple, convenient, high sensitivity than Sanger sequencing method. Scorpion real-time PCR method is suitable for clinical applications.
作者 张余兵
出处 《分子诊断与治疗杂志》 2014年第6期405-409,共5页 Journal of Molecular Diagnostics and Therapy
基金 国家科技部"艾滋病和病毒性肝炎等重大传染病防治专项"(2008ZX10003-012)
关键词 蝎子实时荧光PCR新方法 Sanger测序法 HBV HBV Scorpion real-time PCR method Sanger sequencing method
  • 相关文献

参考文献13

  • 1Andersson K L, Chung R T. Monitoring during and after antiviral therapy for hepatitis B [J]. Hepatology, 2009,49(5Suppl): S 166-173.
  • 2Zheng J X, Zeng Z, Zhang D Y, et al. Prevalence and significance of hepatitis B reverse transcriptase mutants in different disease stages of untreated patients[J]. Liver Int, 2012,32(10):1535-1542.
  • 3Comberg M, Jaroszewicz J, Manns M P, et al. Treatment of chronic hepatitis B [J]. Minerva Gastroenterol Dietol, 2010,56(4):451-465.
  • 4Kew M C. Epidemiology of chronic hepatitis B virus infection, hepatocellular carcinoma, and hepatitis B virus-induced hepatocellular carcinoma[J]. Pathol Biol, 2010,58(4):273-277.
  • 5Jia S, Wang F, Li F, et al. Rapid detection of hepatitis B virus variants associated with lamivudine and adefovir resistance by multiplex ligation-dependent probe amplification combined with real-time PCR[J]. J Clin Microbiol, 2014,52(2):460-466.
  • 6Mu S C, Lin Y M, Jow G M, et al. Occult hepatitis B virus infection in hepatitis B vaccinated children in Taiwan[J]. J Hepatol, 2009,50(2):264-272.
  • 7Goldstein S.T, Zhou F, Hadler S C, et al. A mathematical model to estimate global hepatitis B disease,burden and vaccination impact[J]. International Journal of Epidemiology, 2005,34(6):1329-1339.
  • 8Friedman S L. Mechanisms of hepatic fibrogenesis [J]. Gastroenterology, 2008,134(6):1655-1669.
  • 9Lim S G, Cho S W, Lee Y C, et al. Changes in liver stiffness measurement during antiviral therapy in patients with chronic hepatitis B [J]. Hepatogastroenterology, 2011,58(106):539-545.
  • 10Delaney W E. Progress in the treatment of chronic hepatitis B: long-term experience with adefovir dipivoxil [J]. J Antimicrob Chemother, 2007,59(5):827-832.

二级参考文献3

共引文献4

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部