Hsa-miRNA-9-5p过表达慢病毒载体的构建

Hsa- micrornas- 9- 5p Build Slow Virus Expression Vector

目的深讨Has-miRNA-9-5p过表达慢病毒载体的构建。方法采用相关基因shRNA序列设计、病毒载体松建与鉴定以及病毒包装滴度鉴定、表达检测。结果成功构建慢病毒载体Has-miRNA-9-5p过表达系统。结论为miRNA-9-5p的功能研究奠定基础。

Objective Hsa-miRNA-9-5p to construct Lentivirus Expression Vector. Methods The related gene shRNA sequence,vector construction and identification of virus titer and packaging identification,expression. Results The lentiviral vector was successfully constructed the overexpression of Hsa- miRNA- 9- 5p system. Conclusion Lays the foundation for the functional study of miR- 9- 5p.