盘羊、巴什拜羊及其杂交羊重组ISG15蛋白对体外绵羊肺炎支原体感染的影响

The Effect of Recombinant ISG15 Protein in Argali,Bashbay Sheep and Their Hybrid Sheep Aginst Mycoplasma ovipneumoniae Infection in Vitro

为研究盘羊、巴什拜羊及其杂交羊重组ISG15蛋白对体外绵羊肺炎支原体感染的影响,首先分离试验羊的淋巴细胞,分别将盘羊、巴什拜羊及其杂交羊重组ISG15蛋白和绵羊肺炎支原体加入体外培养的淋巴细胞中共同孵育,利用real-time PCR技术检测肺炎支原体16SrRNA的表达水平。结果表明,体外淋巴细胞与绵羊肺炎支原体、重组ISG15蛋白共培养12h到96h,盘羊和巴什拜羊重组ISG15蛋白组的肺炎支原体的16SrRNA表达水平显著低于对照组(P<0.05),而杂交羊重组ISG15蛋白组的肺炎支原体的16SrRNA表达水平与对照组差异不显著(P>0.05)。说明盘羊和巴什拜羊重组ISG15蛋白在体外可对绵羊肺炎支原体起到抑制作用。

To study the effect of the recombinant ISG15 protein in Argali, Bashbay sheep and their hybrid sheep against Mycoplasma ovipneumoniae （Mo） infection in vitro. First, cultured sheep lymphocytes, and then added the recombinant ISG15 protein of the three breeding sheep and Mo into the cultured lymphocytes respectively, used real-time PCR technology to detect the expression level of Mo 16S rRNA. The results showed that the expression levels of Mo 16S rRNA in Argali and Bashbay sheep group were significant lower than the control goup （P〈0.05）, while the hybrid sheep group had no significant difference with the control group when added the recombinant ISG15 protein for 12 to 96 hours（P〉0.05）. The recombinant ISG15 protein in Argali and Bashbay sheep could inhibit the growth of Mo in vitro.