摘要
OguCMS是甘蓝中应用最为广泛的雄性不育类型,前期表达谱分析表明转录因子BoMYB1在OguCMS花药中表达下调达10.3倍。通过构建酵母双杂交转录因子BD区诱饵载体pGBKT7-BoMYB1,将其转入酵母菌AH109中进行转录因子BoMYB1转录激活功能的分析。结果显示,AH109[pGBKT7-BoMYB1]在SD/-Trp/x-a-gal、SD/-Ade/-Trp/x-a-gal、SD/-His/-Trp/x-a-gal平板上均长出蓝色菌落,表明BoMYB1可以自主激活报告基因ADE2、HIS3和MEL1,说明转录因子BoMYB1具有转录自激活功能。研究结果对于深入探索该基因的功能及阐明OguCMS花药败育的机理具有重要意义。
Ogura cytoplasmic male sterile （OguCMS） is the most widely used male sterile type in cab bage （Brassica oleracea var. capitata）. Early expression pattern analysis indicated thatBoMYBltranscription factor express down regulated 10.3 times in OguCMS anther of cabbage. In this study, the pGBKTT-BoMYB1 bait vector of the Yeast two-hybrid BD region was transformed into the Yeast cell AH109 to analysis the transcriptional activation of BoMYBl transcription factor. Results showed that AHIOg[pGBKT7-BoMYB1] grew blue clonies in SD/-Trp/x-a-gal, SD/-Ade/-Trp/ x-a-gal, and SD/- His/-Trp/x-a-gal plates, which indicatedBoMYB1 could activate yeast reporter genes ADE2, HIS3and MELlautonomously. So the self-activation of BoMYBlwas illustrated. The experimental results are important for exploring the function of BoMYB1 deeply and illuminating the mechanism of OguCMS anthers abortion.
出处
《西北农业学报》
CSCD
北大核心
2014年第10期120-126,共7页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家自然科学基金(31372065)
“十二五”国家科技支撑计划(2012BAD02B01,2012BAD50G01)
863项目(2012AA100105,2012AA020103)
