利多卡因对肺缺血再灌注损伤模型大鼠炎症反应因子的影响

Effects of Lidocaine on Inflammatory Factor in Lung Ischemia-reperfusion Model Rats

目的:研究利多卡因对肺缺血再灌注损伤模型大鼠炎症反应因子的影响。方法:取90只大鼠随机均分为假手术组、模型组和预处理(1 mg/kg)组,除假手术组外其余各组大鼠建立左肺缺血再灌注损伤模型,预处理组大鼠建模前15 min注射利多卡因。各组大鼠均于缺血30 min、再灌注60 min、再灌注120 min时采集标本,检测支气管肺泡灌洗液的蛋白总量(TP)、白细胞(WBC)数目、中性粒细胞(PMN)比例,肺组织的湿/干质量(W/D)比值及其组织匀浆中肿瘤坏死因子α(TNF-α)、白细胞介素(IL)-1、IL-6和IL-8的含量。结果:与假手术组比较,模型组大鼠再灌注60、120 min时和预处理组再灌注120 min时TP、WBC数量、PMN比例、W/D比值及肺组织匀浆中TNF-α、IL-1、IL-6及IL-8含量均明显升高,差异具有统计学意义(P<0.05);与模型组比较,预处理组大鼠再灌注60、120 min时TP、WBC数量、PMN比例、W/D比值及肺组织匀浆中TNF-α、IL-1、IL-6及IL-8含量均明显降低,差异具有统计学意义(P<0.05)。结论:利多卡因可能通过抑制免疫反应改善大鼠肺缺血再灌注损伤。

OBJECTIVE： To study the effects of lidocaine on inflammatory factor in lung ischemia-reperfusion model rats. METHODS： 90 rats were randomly divided into sham operation group, model group and pretreatment group （1 mg/kg）. Left lung ischemia-reperfusion model was established in those groups except for sham operation, and pretreatment group was given lidocaine intravenously 15 min before modeling. The samples were collected 30 min after isehemia, 60 rain and 120 min after reperfusion. TP, WBC and PMN proportion of bronchoalveolar lavage fluid, W/D, the contents of TNF-α, IL-1, IL-6 and IL-8 in tissue homogenate were determined. RESULTS： Compared with sham operation group, TP, WBC and PMN proportion of bronchoalveolar lavage fluid, W/D, the contents of TNF-α, IL-1, IL-6 and IL-8 in lung tissue homogenate were increased significantly 60 and 120 min after reperfusion in model group and 120 min after reperfusion in pretreatment group; there was statistical significance （P〈 0.05）. Compared with model group, TP, WBC, PMN proportion, W/D, the contents of TNF-α, IL-1, IL-6 and IL-8 in lung tissue homogenate were decreased significantly in pretreatment group 60 and 120 min after reperfusion; there was statistical significance （P〈0.05）. CONCLUSIONS： Lidocaine might improve lung ischemia-reperfusion injury through inhibiting immunological reaction.