4Zhao ZL,Ma X,Xu MQ,et al.Protein cyclization enhancedthermostability and exopeptidase-resistance of green fluorescentprotein[J].J Microbiol Biotech,2010,20(3):460-466.
6Zhao ZL,Lu W,Xu Ming-Qun,et al.Purification of greenfluorescent protein using a Two-Intein system[J].Appl Mi-crobiol Biotech,2008(77):1175-1180.
7Sambrook,Russell.分子克隆实验指南(第三版)[M].北京:科学出版社,2008.
8Crameri A,Whitehorn EA,Tate E.Improved green fluorescentprotein by molecular evolution using DNA shuffling[J].NatureBiotechnol,1996,14:315-319.
9Andersen JB,Sternberg C,Poulsen LK,et al.New unstablevariants of green fluorescent protein for studies of transient geneexpression in bacteria[J].Appl Environ Microbiol,1998,64:2240-2246.
10Cormack BP,Valdivia RH,Falkow S.FACS-optimized mu-tants of the green fluorescent protein(GFP)[J].Gene,1996,173:33-38.
