龙须菜新品系ZC的生长和生理生化参数的测定及其SCAR标记的开发

The Determination of Growth,Physiological,Biochemical Parameters and the Development of SCAR Marker of the New Strain ZC of Gracilariopsis lemaneiformis(Rhodophyta)

对龙须菜新品系ZC与栽培品系981、07-2及湛山湾野生四分孢子体的生长速度和总蛋白质含量等生理生化参数进行比较,从而为新品系ZC在栽培产业的开发利用提供理论支持。结果显示,ZC的线性生长速度高达9.89mm/d,比栽培品系981、07-2分别提高16.6%、51.7%,表明ZC相比现有的栽培品系具备较高的生长速度。藻红蛋白含量与981无明显不同,但显著低于07-2,叶绿素a含量则显著低于2个栽培品系;其总蛋白质含量7.21mg/g显著高于其他3个龙须菜材料,可作为一种提供更高蛋白质来源的鲍鱼饵料使用。在光合放氧和呼吸耗氧速率方面,ZC并未与其他龙须菜材料表现出显著差异。另外,由于ZC在外观形态上与栽培品系很难区分,本实验通过分析上述4种龙须菜的RSAP指纹图谱,筛选获得了ZC的特异条带,并将其转化为稳定可靠、特异性高的SCAR标记,用以鉴定龙须菜新品系。

Quantitative traits of growth rate, physiological and biochemical parameters including total protein content, pigment contents and the rates of photosynthetic oxygen evolution and respiration oxy- gen consumption were determined and measured among four strains of Gracilariopsis lernaneiformis, one of which was collected in Zhanshan Bay, Qingdao, the other two strains were 981 and 07-2 culti- vars, and the new strain named ZC. The aim of the study was to provide assistant evidence for cultiva- tion application of this strain. The linear growth rate of the new strain was up to 9.89 mm/d and increased by 16.6%,51.7% more than that of 981 and 07-2 respectively, which illustrated that growth rate of the new strain was higher than the existing cultivars. Its phycoerythrin content was significantly lower than 07-2 and had no significant differences with 981. However, its chlorophyll a content was markedly lower than two cultivars. Total protein content （7.21 rag/g） of the new strain was the highest among four strains, which demonstrated that it could be a source as abalone bait for higher protein. There were no evident differences between the new strain and other materials of G. lemaneiformis in the rates of photosynthetic and respiration. In addition, as the new strain was morphologically similar to cultivar, RSAP finger printing was compared among four strains, specific band of the new strain was screened and converted into sequence characterized amplified regions （SCAR） molecular markers with high stability and specificity to identify the new strain.