枣转录因子ERF 3′端序列克隆及生物信息学分析

Cloning and Bioinformatic Analysis of the 3′-end of ERFGene in Ziziphus jujuba

以枣为试材,根据GenBank中登录的苹果、拟南芥等植物EFR保守序列设计引物,采用同源克隆法克隆出该基因3′端序列并对其编码的蛋白进行了生物信息学分析。结果表明:该试验克隆获得1条长度为959bp的条带;经过序列分析,该序列包含典型的AP2/EREBP保守结构域,与桑树、欧洲山毛榉和欧洲栗ERF基因序列相似度分别为79%、78%、77%;系统进化树分析显示,枣的ZjERF与可可树、陆地棉和海岛棉的AP2/EREBP亲缘关系较近,属于ERF家族的B2亚群;此外,还用Swiss Model程序(http://au.expasy.org/tools/)预测了ZjERF基因编码蛋白的三维结构。

Taking Ziziphus jujubaas material,the fragment of ERFgene contained 3′-end of ZjERFwas first isolated by homologous gene cloning method on the basis of the homologous genes of Malus domestica,Arabidopsis thaliana and others in GenBank.The results showed that a 959 bp fragment was amplified.Conservation analysis of ZjERFshowed that it had a typical zone of AP2/EREBP;similarity analysis showed that the protein had highly homologous to the ZjERFof Morus alba,Fagus sylvatica and Castanea sativa with homologous of 79%,78% and 77% respectively;Phylogenic analysis showed that ERFof species in jujube had a closer relationship with Theobroma cacao,Gossypium hirsutum,Gossypium barbadense and ZjERF blonged to B2 group of ERFfamily.Tertiary structure of ZjERF was speculated by Swiss Model program（http：//au.expasy.org/tools/）.