Lipozyme TL 100L的固定化及其催化甘油解制备甘油二酯的研究

Immobilization of Lipozyme TL 100L and its application in synthesis of diglycerides by glycerolysis

利用4种常用大孔树脂(NKA-9,AB-8,D301R,D4020)对Lipozyme TL 100L进行固定化,并比较了固定化效果及4种固定化脂肪酶对甘油解反应制备甘油二酯的影响。结果表明:以弱极性大孔吸附树脂AB-8为载体固定化的Lipozyme TL 100L在无溶剂体系中催化大豆油甘油解反应制备甘油二酯的效率最高;当底物(精炼大豆油与甘油)摩尔比为1∶2,反应温度为50℃,AB-8大孔树脂固定化的Lipozyme TL 100L加酶量为600 U/g时,在甘油解反应6 h后产物中甘油二酯含量基本达到平衡,为54.39%,其中1,2-甘油二酯含量为33.87%,1,3-甘油二酯含量为20.52%。

Lipozyme TL 100L was immobilized by four kinds of common macroporous resins（ NKA -9, AB -8, D301 R, D4020）. The immobilization effects of the four kinds of common macroporous resins were compared, and the impacts of four kinds of the immobilized lipases on synthesis of diglycerides by glycerolysis were also compared. The results showed that immobilized Lipozyme TL 100L with macroporous resin AB -8 as carrier had the highest catalytic efficiency in synthesis of diglycerides by glycerolysis of soybean oil in a solvent free system. When dosage of immobilized Lipozyme TL 100L with macroporous resin AB - 8 as carrier was 600 U/g, molar ratio of refined soybean oil to glycerol was 1 ： 2 and reaction temperature was 50 ℃, the content of diglyeerides in the product reached 54.39% after glycerolysis for 6 h, in which the contents of 1,2 - diacylglycerol and 1,3 - diacylglycerol were 33.87% and 20.52% respectively.