梗阻性黄疸患者胆汁微生物群落限制性片段多态性分析

Microbial community structure in bile from obstructive jaundice by terminal restriction fragment length polymorphism

目的 探讨梗阻性黄疸患者胆汁中的微生物群落结构.方法 应用末端标记限制性片段长度多态性（T-RFLP）和克隆文库分析,对昆明医科大学第二附属医院肝胆胰外科三病区2010年10月至2013年10月期间诊断为梗阻性黄疸患者的胆汁进行细菌培养,培养结果为阴性的患者胆汁再进行微生物群落结构进行分析.结果 共117例患者的胆汁纳入研究,胆汁中细菌16S核糖体DNA （rDNA）阳性率为42.7％ （50/117）.不同梗阻原因胆汁中细菌16S rDNA阳性率（97.3％比17.5％）差异有统计学意义（P＜0.05）,而梗阻位置相同的细菌16S rDNA阳性率（43.3％比38.1％）差异无统计学意义（P＞0.05）.结论 采用T-RFLP方法分析16S rDNA克隆片段能够有效评估梗阻性黄疸患者胆汁中的细菌群落存在和多样性.

Objective To determine the microbial community structure in bile from obstructive jaundice.Methods Structure of bile microbial community from obstructive jaundice patents was investigated by terminal restriction fragment length polymorphism （T-RFLP）and clone libraries approaches.16S ribosomal DNA （16S rDNA） was retrieved from the patients with obstructive jaundice and negative bile culture from October 2010 to October 2013 in our ward.Results 117 cases of patients included in the study of bile.According to percentage of 16S rDNA stone from 50 patients with negative bile culture.The positive rate of bacterial 16S rDNA was 42.7% （50/117）.The positive rate of bacterial 16S rDNA have different reasons for biliary obstruction in a significant difference （97.3% vs.17.5%）,but there is no statistical difference between the position of obstruction （43.3% vs.38.1%）.Conclusion The result illuminated that T-RFLP analysis of cloned 16S rDNA fragments is a powerful tool for estimating the community an diversity of bacterial in bile sample from obstructive jaundice.