内皮抑素基因联合骨髓间充质干细胞治疗大鼠脑胶质瘤

Endostatin gene combined with bone marrow mesenchymal stem cells for treating brain glioma in rats

目的 探讨联合应用内皮抑素（ES）基因和骨髓间充质干细胞（BMSCs）治疗胶质瘤的有效性.方法 建立大鼠胶质瘤脑内模型;将转染了ES基因真核表达质粒的BMSCs注入模型鼠脑内,设立对照组,通过4组大鼠生存期、第6、12、18和24天肿瘤体积、病理切片、电镜检查、肿瘤坏死率及血管计数,比较各组治疗的差异.结果 大鼠生存期A组明显高于其他各组（P＜0.01）;肿瘤体积在第12天后,A组[（107.12±14.32） mm3]明显小于其他各组（P＜0.05）;A组肿瘤细胞凋亡较其他组明显;肿瘤坏死率A组（33.7％）明显高于其他各组（P＜0.01）;血管计数A组（5.73±0.02）明显少于其他各组（P＜0.01）.结论 应用内皮抑素基因联合BMSCs可有效治疗胶质瘤.

Objective To discuss the effectiveness of endostatin gene combined with bone marrow mesenchymal stem cells （BMSCs） in treating brain glioma.Methods The rat glioma model in the brain was established.Four groups were set up randomly.The difference in curative effect among groups was compared by life span,tumor volume at 6th,12th,18th and 24th day,HE staining,transmission electron microscope and necrosis ratio.Results In group A,life span was extended obviously as compare with other groups （P 〈0.01）.Tumor volume in group A [（107.12 ± 14.32） mm3] was diminished obviously as compared with group B [（395.47 ± 66.48） mm3] and group C [（382.74 ± 48.58） mm3,P 〈 0.05] at 12th day,which was diminished significantly as compared with group D [（539.45 ± 39.45） mm3,P 〈 0.01].Apoptosis was increased obviously in group A.Necrosis ratio and vessel counts in group A （33.7%,and 5.73 ±0.02） were obviously greater than other groups （P 〈0.01）.Conclusion Endostatin gene combined with bone marrow BMSCs can treat glioma effectly.