糖皮质激素诱导蛋白激酶1基因在肺癌组织中的表达及其临床意义

Expression of glucocorticoid-induced protein kinase 1 gene in lung carcinoma and its clinical significance

目的 观察人肺癌组织中糖皮质激素诱导蛋白激酶1（SGK1）基因的表达.方法 应用逆转录-聚合酶链反应（RT-PCR）方法检测10例中晚期肺癌及其癌旁组织中SGK1 mRNA的相对表达量,并采用Western blot检测上述组织中SGK1蛋白表达.结果 70％（7/10）的肺癌组织中SGK1 mRNA和蛋白表达下调或缺失,而癌旁组织中80％ （8/10）该基因表达上调和蛋白表达增加,两者差异有统计学意义（P＜0.05）.结论 肺癌组织中SGK1表达下调,可能在肺癌的发生、发展过程中发挥重要作用.

Objective To observe the expression of glucocorticoid-indueed protein kinase 1 （ SGK1 ） gene in human lung carcinoma. Methods The samples were obtained from 10 cases of lung carcinoma. Reverse transcription-polymerase chain reaction （ RT-PCR ） and Western blotting were used for analyzing the SGK1 mRNA and protein expression in 10 samples and adjacent noncanerous tissues. Results SGK1 mRNA and protein expression was not detected in 70% of human lung carcinoma and 20% adjacent noncanerous tissues （P 〈 0.05 ）. Conclusion SGK1 expression is downregulated in human lung carcinoma tissue, which may play some roles in the pathogenesis of human lung carcinoma.