苹果梨果实CHI基因cDNA克隆及表达分析

Molecular Cloning and Expression Analysis of Chalcone Isomerase Gene from Pingguoli Fruit

以苹果梨果实为试验材料,克隆得到CHI基因c DNA序列(片段),Gen Bank登录号:JN120854,其长度为444 bp,推断其编码148个氨基酸序列。生物信息学分析结果表明,该氨基酸序列与其他植物CHI氨基酸序列的同源性在90%左右,与砂梨CHI氨基酸序列聚类关系最近,该CHI基因c DNA序列含有常用的限制性内切酶Bam HI的识别位点。半定量RT-PCR分析结果表明,随着果实的成熟,套袋果与未套袋果CHI基因的表达量都不断增加,但套袋果在果实成熟期的表达量明显高于未套袋果。

Pingguoli fruit was used as material in the experiment,CHI gene cDNA sequence （fragment） was cloned,accession number：JN120854,partial sequence was 444 basepair,coding 148 amino acids.Bioinformatics analysis results show that CHI amino acid sequence homology is 90％ with other plant,and CHI amino acid of the Pingguoli had closer relationship with Shali,there was a commonly used restriction enzyme site for BamHI from CHI gene cDNA sequence.The semi-quantitative RT-PCR technique showed that as fruit mature,expression of CHI gene is increasing constantly about bagged fruit and not bagged fruit,but bagged fruit of expression obviously higher than not bagged fruit in the fruit mature.