玉米种质材料遗传多样性的ISSR分析

Genetic Diversity of Maize Accessions Using Inter-simple Sequence Repeat(ISSR) Markers

[目的]明确山西主要玉米品系的主要遗传多样性特点。[方法]利用ISSR分子标记技术,对供试的玉米种质资源进行遗传多样性分析。[结果]用筛选出的9个多态性高、重复性好的引物对供试种质材料进行扩增,共扩增出清晰条带74条,多态性条带56条,多态性条带比率为75.7%。应用DPS软件对试验结果进行聚类分析,构建聚类分析树状图,在遗传相似系数为0.55处,将供试种质材料分为5个类群。[结论]为今后山西玉米育种与杂交亲本的选择提供理论依据。

[Objective] The aim was to clear main genetic diversity characteristics of main corn strain in Shanxi.[Method] Using ISSR molecular markers to analyze the genetic diversity of maize germplasm resources.[Result] Nine primer of high polymorphism and good repeatability were used to amplify the germplasm material on the test.74 bands were amplified,including 56 polymorphism bands.The percentage of polymorphism bands was 75.7％.Application of DPS software clustering analysis was carried out on the experimental results,and built the cluster analysis tree.The test germplasm materials were divided into five groups at the genetic similarity coefficient of 0.55.[Conclusion] The study provided theoretical reference for the selection of hybrid parent and maize breeding in the future.