不同贝母的指纹图谱鉴别研究

The Identification of Different Fritillaria Species by Fingerprints

[目的]建立指纹图谱方法,区分与鉴别浙贝母、平贝母、川贝母药材。[方法]采用高效液相色谱法,蒸发光散射检测器。[结果]测定方法稳定可靠,3种贝母测定精密度、重复性RSD均小于5%,同时色谱峰均具有各自的特征性。[结论]该方法稳定可靠,可作为浙贝母、平贝母、川贝母区分与判定的有效途径。

[Objective] To establish a fingerprint determination method to distinguish and identify Fritillariae thunbergii bulbus,Fritillariae cirrhosae bulbus,Fritillariae ussuriensis bulbus.[Method] HPLC and ELSD were adopted.[Result] The method is reliable,the reproducibility and precision of Fritillariae thunbergii bulbus,Fritillariae cirrhosae bulbus,Fritillariae ussuriensis bulbus are all less than 5％,and the chromatographic peaks are characterized.[Conclusion] This method is stable and reliable,it can be a good method to distinguish and identify Fritillariae thunbergii Bulbus,Fritillariae cirrhosae bulbus,Fritillariae ussuriensis bulbus.