西藏垫状点地梅总生物碱提取工艺研究

Reseach on the Extraction Technology of Total Alkaloids from Androsace tapete in Tibet

[目的]研究垫状点地梅生物碱热回流提取的较优方法。[方法]采用热回流提取、单因素对比试验、正交试验和紫外-可见光分光光度分析,提出了垫状点地梅生物总碱的合理提取方案。[结果]乙醇体积分数75%、回流温度75℃、料液比(m/v)1∶15、回流时间3h为最佳提取条件,此时提取垫状点地梅生物总碱提取率将达1.257%。[结论]该试验方法简便、科学可靠,可对垫状点地梅生物碱工业提取起到一定的参考价值。

[Objective] The research aimed to study the optimum method of alkaloids hot reflux extraction from Androsace tapete.[Method]The alkaloids reasonable extraction scheme of Androsace tapete was proposed by heat reflux extraction,single factor comparative experiment,orthogonal experiment and UV-Vis spectrophotometric analysis.[Result] While the volume fraction of ethanol was 75％,the refiux temperature was 75 ℃,the ratio of material to liquid（m/v） was 1∶15,and the refiux time was 3 hours,we can get the best extraction effect to the total Alkaloids of Androsace tapete.The extraction rate of alkaloids from Androsace tapete would reach 1.257％.[Conclusion] This experimental method was simple,scientific and reliable,and can have a certain reference value for the industrial extraction of alkaloids from Androsace tapete.