高效液相色谱-四级杆飞行时间串联质谱的儿童性早熟代谢组学研究

Metabolomics study on central precocious puberty using HPLC-QTOF-MS analysis

目的用代谢组学研究儿童真性性早熟(CPP)的代谢标记物,以探讨儿童性早熟的发病机制。方法用高效液相色谱-四级杆飞行时间质谱联用系统(HPLC-QTOF-MS)对性早熟组和对照组儿童的血液样本进行代谢图谱分析,并结合主成分分析法(PCA)和偏最小二乘辨别分析法(PLS-DA),根据PLS分析的载荷图和VIP值,筛选24个代谢标记物,并对其参与的代谢途径进行分析。结果氨基酸、5-羟色胺、儿茶酚胺及脂代谢等多条代谢途径异常与儿童性早熟发生相关。此外,一些环境内分泌干扰物也可能导致性早熟。结论性早熟儿童和正常儿童血液代谢特征存在明显差异。

Objective To apply metabolomics studies to find out meta-bolic markers for further understanding the pathogenesis of children cen-tral precocious puberty （ CPP ）.Methods A high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrom-etry method （ HPLC-QTOF-MS） was used to figure out the metabolic characteristic of the CPP children and healthy children.Combined with the principal component analysis （ PCA） and partial least squares-diac-riminant analysis （ PLS-DA） , the major metabolic changes in peripher-al blood between the normal and onset group were statistically analyzed.According to the loading plot and variable importance plot （ VIP ） of PLS, twenty-four metabolic markers were identified.Furthermore, the pathways mediated by these metabolic markers were analyzed.Results CPP is closely related to the abnormalities of multiple metabolic path-ways, including amino acid, catecholamine, serotonin, and lipid metab-olism.Additionally, environmental endocrine disruptors play an impor-tant role in the occurrence of CPP.These findings provide a novel insight to the pathogenesis of CPP.Conclusion The metabolic characteristic of the CPP children was obviously different from that of the normal children.