银杏黄酮苷元对氧化低密度脂蛋白所致内皮细胞炎症损伤的影响

Influence of ginkgo flavone aglycones on oxidized low-density lipoprotein induced inflammatory injury of endothelial cells

目的：观察银杏黄酮苷元（ GA）对氧化低密度脂蛋白（ ox－LDL）诱导人主动脉内皮细胞（HAECs）细胞间粘附分子－1（ICAM－1）、血管细胞粘附分子－1（VCAM－1）、单核细胞趋化蛋白－1（MCP－1）的影响及核转录因子－κB（NF－κB）在其中的调控作用。方法体外培养HAECs，建立HAECs的ox－LDL损伤模型前，用不同浓度GA预处理细胞1 h，用四甲基噻唑兰法检测内皮细胞活性，免疫荧光法检测细胞核内NF－κB的激活，细胞酶联免疫吸附法检测MCP－1、ICAM－1及VCAM－1水平。结果30～60 mg·L^－1 GA预处理组显著抑制ox－LDL诱导的内皮细胞NF－κB激活（ P＜0.05），下调VCAM－1、ICAM－1及MCP－1表达（ P＜0.05）。结论 GA能够有效抑制ox－LDL诱导HAECs炎症因子ICAM－1、VCAM－1、MCP－1表达。

Objective To observe the influence of ginkgo flavone agly-cones （ GA） on oxidized low-density lipoprotein （ ox-LDL） induced in-tercellular cell adhesion molecule 1 （ ICAM-1 ） , vascular cell adhesion molecule 1 （ VCAM-1 ） , monocyte chemotactic protein 1 （ MCP-1 ） in human aortic endothelial cells （ HAECs） and the mediated role of neclear factor-kappa B （ NF-κB）.Methods Cultured HAECs in vitro were pretreated with different concentrations GA for one hour and then ox -LDL injury model of HAECs were established.MTT assay was used to detect endothelial cell activity, immunofluorescence was used to detect NF-κB activation, and enzyme-linked immunosorbent assay （ ELISA） was used to detect MCP-1, ICAM-1 and VCAM-1 level.Results GA 30-60 mg ·L^-1 pre-treatment group significantly inhibited ox-LDL-induced endothelial cells NF -κB activation （ P 〈0.05 ） and lowered the expression of VCAM -1 , ICAM -1 and MCP -1 （ P 〈0.05）.Conclusion GA can effectively inhibit the expressions of ox-LDL -induced HAECs inflammatory factors, such as ICAM -1, VCAM-1 , and MCP-1.