甜瓜持绿蛋白的生物信息学分析

Bioinformatics Analysis of Stay-green Proteins in Melon

采用生物信息学方法,对甜瓜持绿蛋白(stay-green protein,SGR)理化性质、信号肽、前导肽、功能型位点、疏水性和亲水性、跨膜结构域、蛋白质二级结构和三级结构进行预测和分析,并构建系统进化树.结果表明:甜瓜含有4个持绿蛋白家族成员,进化分析发现有2个同源基因对,同源的基因对之间功能上完全冗余,4个蛋白都不具有信号肽和跨膜结构域,属于非分泌型蛋白,推测SGR可能在细胞质基质中发挥功能.采用同源建模法预测其三级结构,并通过拉氏构象图分析准确性和合理性,证明了同源建模的结果相对可靠性.

Using bioinformatics methods, the physical and chemical properties, signal peptide, leader peptide, functional sites, hydrophobicity and hydrophilicity, trans-membrane topological structure,the secondary structure, the tertiary structure of the melon （Cucurnis melo L. ） staygreen proteins were predicted and analyzed. Phylogenetic trees were constructed. Results showed that the melon stay-green protein （ SGRs ） family contains four members. Two homologous genes were found by phylogenetic analysis and predicted fully redundant in function. The four proteins have no signal peptide and transmembrane domain, belong to non-secretory protein,and supposed to play a function in the cytoplasmic. The accuracy and reasonableness were analyzed with a tertiary structure prediction by homology modeling and ramachandran diagram. The results demonstrate the relative reliability of the homologous modeling result.