右美托咪定对烫伤大鼠细胞免疫功能的影响

Effects of dexmedetomidine on the cellular immune function of rats with scald

目的 评价右美托咪定对烫伤大鼠细胞免疫功能的影响.方法 健康雄性SD大鼠72只,体重200 ～ 220 g,日龄120～ 150 d.采用随机数字表法,将其分为3组（n=24）：正常对照组（C组）、烫伤组（S组）、右美托咪定组（D组）.大鼠背部区域脱毛后浸入94℃热水中12 s,制备30％体表面积烫伤模型.C组不做任何处理,S组制备烫伤模型后进行容量复苏治疗,D组制备烫伤模型后除进行容量复苏治疗外,同时于制备模型后即刻腹腔注射右美托咪定30μg/kg.于模型制备前（T1）、烫伤后12 h（T2）、24 h（T3）时采集下腔静脉血,测定T淋巴细胞亚群CD3＋、CD4＋、CD8＋和NK细胞、C反应蛋白（CRP）、IL-6、IL-10及TNF-α的水平,计算CD4＋/CD8＋比值,并取股动脉血,进行血气分析.结果 与C组比较,S组和D组T2.3时CD3＋、CD4＋、CD4＋/CD8＋比值、pH值、PaCO2、PaO2、NK细胞水平降低,CD8＋、IL-6、IL-10、TNF-α及CRP水平升高,BE负值增加（P＜0.05）.与S组比较,D组T23时CD3＋、CD4＋、CD4 ＋/CD8＋比值、IL-10、pH值、PaCO2、PaO2、NK细胞水平升高,CD8＋、IL-6、TNF-α及CRP水平降低,BE负值减少（P＜0.05）.结论 右美托咪定可改善烫伤大鼠细胞免疫功能.

Objective To evaluate the effects of dexmedetomidine on the cellular immune function of the rats with scald.Methods Seventy-two healthy male Sprague-Dawley rats,weighing 200-220 g,aged 120-150 days,were randomly divided into 3 groups （n =24 each）：normal control group （group C）,scald group （group S） and dexmedetomidine group （group D）.Thirty percent of the total body surface was shaved and then exposed to 94 ℃ water for 12 s in S and D groups.The rats were resuscitated according to Parkland formula after scald in S and D groups,and in addition,dexmedetomidine 30 μg/kg was also intraperitoneally injected immediately after scald in D group.Before the model was established （T1） and at 12 and 24 h after scald （T2,3）,blood samples from the inferior vena cava were collected for determination of T lymphocyte subsets CD3 ＋,CD4 ＋ and CD8 ＋,NK cell,C-reactive protein （CRP）,interleukin-6 （IL-6）,IL-10 and tumor necrosis factor-alpha （TNF-α） level.CD4＋/CD8＋ was calculated.Arterial blood samples were collected for blood gas analysis.Results Compared with C,the CD3＋,CD4＋ and NK cell levels,CD4＋/CD8＋,pH value,PaCO2 and PaO2 were significantly decreased,and CD8＋ levels,IL-6,IL-10,TNF-α,CRP and BE negative value were increased at T2,3 in S and D groups.Compared with group S,the CD3＋,CD4＋,NK cell and IL-10 levels,CD4＋/CD8＋,pH value,PaCO2 and PaO2 were significantly increased,and CD8＋ levels,IL-6,TNF-α,CRP and BE negative value were decreased at T2,3 in group D.Conclusion Dexmedetomidine can improve the cellular immune function of the rats with scald.