β-环糊精结合响应面法优化茶多酚的提取工艺

Optimization of the extraction process of tea polyphenols from tea dust by response surface methodology and β-CD

以β-环糊精为提取介质,蒸馏水为提取溶剂,从制茶副产物——茶末中提取多酚类物质。在单因素试验基础上,采用Box-Behnken试验设计原理和响应面分析法研究了β-环糊精含量、提取温度和料液比对茶多酚提取率的影响,并建立该工艺的二次多项式模型。结果表明,回归模型具有高度显著性,可以对茶多酚得率进行很好地分析和预测;确定了茶多酚提取工艺的最佳条件为:β-环糊精含量8.41%、提取温度58℃、料液比1:57(g/mL),且在此条件下,茶多酚得率的试验值为27.96%,与模型预测值28.06%只相差了0.1%。与传统的水回流法和超微粉碎法相比,β-环糊精辅助提取法提取率最高。

The tea polyphenols was extracted from tea dust with β-CD as extraction medium and distilled water as extraction agent. Based on the single factor experiment, the effects of β-CD content, extraction temperature and material-to-solvent ratio on the extraction efficiency of tea polyphenols were investigated by the Box-Behnken experiment design principle and the response surface analysis and a quadratic polynomial model was established and validated to be highly significant and allow the prediction of the extraction efficiency of tea polyphenols. The optimum conditions for the extraction of tea polyphenols were as follows： β-CD content of 8.41%, extraction temperature of 58 ℃, solid-to-solvent ratio of 1：57（g/mL）. Under these conditions, the extraction efficiency of tea polyphenols was 27.96%, which was close to the predicted value of 28.06%. The tea polyphenols yield by the β-CD assisted extraction is more than the traditional boiling reflux method and ultrafine grinding extraction technology.