博落回内生真菌的分离及产血根碱菌株的筛选

Isolation of endophytic fungi from Macleaya cordata and screening of sanguinarine-producing strains

采用组织块法从皖西大别山野生博落回Macleaya cordata中分离内生真菌,根据形态学特征对其进行类型归类,采用薄层色谱(TLC)和高效液相色谱(HPLC)鉴定其内生真菌次级代谢产物,运用形态学方法对活性菌株进行初步菌种鉴定。实验结果显示,分别从博落回根、茎、叶中分离出28,57,96株内生真菌。根据形态学特征,初步把其归类为26个类型。其中菌株BLH 51的发酵液中含有血根碱,经形态学初步鉴定为层出镰刀菌Fusarium proliferatum。表明药用植物博落回组织中含有较丰富的内生真菌,可以成为寻找生物活性代谢产物的一个新来源。

Endophytic fungi were isolated from Macleaya cordata growing in Dabie Mountain by agar-block method,and then the endophytic fungi were grouped into different types based on their morphological characteristics,and thin layer chromatography（ TLC） and high performance liquid chromatography（ HPLC） were employed to determine whether the metabolic substances contained sanguinarine or not,and then preliminarily identified by morphological method. The results showed that the leaves hosted the largest number of endophytes（ 96 isolates） followed by the stems（ 57 isolates） and finally the roots（ 28 isolates）,respectively. Based on morphological characteristics the endophytic fungi were grouped into 26 types in our study. TLC and HPLC results showed that there was sanguinarine in the metabolic substances of BLH 51 strain. According to the morphological characteristic,the BLH 51 strain was identified as Fusarium proliferatum. All these indicated that the medicinal plant M. cordata harbors abundant endophytes,which could be a new source for the search of active secondary metabolites.