杜仲不同炮制品化学成分研究

Investigation on chemical constituents of processed products of Eucommiae Cortex

根据2010年版《中国药典》制备生杜仲、盐杜仲、杜仲炭3个规格炮制品,按照2010年版《中国药典》标准对各炮制品进行各项指标检测,均符合药典要求。运用HPLC-DAD对生杜仲、盐杜仲、杜仲炭及杜仲叶中化学成分进行比较分析,结果显示:1杜仲皮主要含有木脂素类化合物松脂醇二葡萄糖及环烯醚萜类化合物京尼平、京尼平苷、京尼平苷酸,而杜仲叶主要含有黄酮类化合物槲皮素和酚酸类化合物绿原酸;2杜仲皮中松脂醇二葡萄糖含量约是杜仲叶中含量的18倍,盐炙和炒炭后松脂醇二葡萄糖质量分数约分别下降30%,85%;3杜仲皮中京尼平、京尼平苷和京尼平甘酸含量约为杜仲叶中京尼平、京尼平苷和京尼平甘酸含量的3,23,28倍,盐炙后京尼平、京尼平苷和京尼平甘酸质量分数分别降低25%,40%,40%,炒炭后京尼平、京尼平苷和京尼平甘酸质量分数分别降低98%,70%,70%;4杜仲皮中咖啡酸含量约是杜仲叶中咖啡酸含量的3倍,盐炙后咖啡酸质量分数约下降50%,炒炭后咖啡酸质量分数下降约75%;杜仲皮中绿原酸含量约为杜仲叶中绿原酸含量的1/6,盐炙和炒炭后绿原酸质量分数分别下降40%,75%;杜仲皮中槲皮素含量仅为杜仲叶中1/40,盐炙和炒炭后槲皮素质量分数分别降低60%,50%。

According to the 2010 Chinese pharmacopeia,salt processed and charcoal processed Eucommiae Cortex were prepared. HPLC-DAD analysis of the content of the bark and leaf of Eucommiae Cortex showed that the bark of Eucommiae Cortex mainly contained lignans such as pinoresinol glucose and iridoid including genipin,geniposide,geniposidic acid,while the leaf of Eucommiae Cortex consisted of flavonoids such as quercetin and phenolic compound such as chlorogenic acid. The content of pinoresinol diglucoside in the bark of Eucommiae Cortex was about 18 times more than that in the leaf of Eucommiae Cortex. The content of pinoresinol diglucoside in salted and charcoal processed Eucommiae Cortex decreased approximately by 30% and 85%,respectively. The content of genipin,geniposide and geniposidic acid in the bark of Eucommiae Cortex was about 3 times,23 times,28 times more than that in the leaf of Eucommiae Cortex. The content of genipin,geniposide and geniposidic acid in salted Eucommiae Cortex were reduced by25%,40% and 40%,respectively. The content of genipin,geniposide and geniposidic acid in charcoal processed Eucommiae Cortex were reduced by 98%,70%,70%,respectively. The content of caffeic acid in bark of Eucommiae Cortex was about 3 times more than that in the leaf of Eucommiae Cortex. The content of caffeic acid was decreased by about 50% in the salted Eucommiae Cortex.While the content of caffeic acid in charcoal processed Eucommiae Cortex was decreased approximately 75%; the content of chlorogenic acid in bark of Eucommiae Cortex was about 1 /6 of that in the leaf of Eucommiae Cortex. The content of chlorogenic acid in salted and charcoal processed Eucommiae Cortex decreased by 40% and 75%,respectively; the content of quercetin in bark of Eucommiae Cortex was only 1 /40 of that in the leaf of Eucommiae Cortex. The content of quercetin in salted and charcoal processed Eucommiae Cortex were reduced by 60% and 50%,respectively.