RP-HPLC-PDA同时测定石见穿不同部位中5个三萜酸

Determination of five triterpenic acids from Salvia chinensis of different parts by RP-HPLC-PDA

目的：建立RP-HPLC-PDA测定石见穿不同部位中5个三萜酸（坡模酸、山楂酸、科罗索酸、齐墩果酸和熊果酸）的定量方法。方法：采用Kromasil C18色谱柱（4.6 mm×250 mm,5μm）,流动相为乙腈-水（90∶10,体积比）,等度洗脱,检测波长205 nm,流速0.8 m L·min^-1,柱温28℃。结果：坡模酸、山楂酸、科罗索酸、齐墩果酸和熊果酸线性范围分别为0.096 00-2.400,0.123 0-3.075,0.242 0-6.050,0.283 0-7.075和0.273 0-6.825μg（r=0.999 8,0.999 7,0.999 9,0.999 5,0.999 9）,平均加样回收率（n=6）分别为98.43%,98.13%,100.6%,98.19%,99.15%（RSD 1.3%,0.67%,1.2%,0.87%,0.43%）。结论：该方法操作简便,重复性好,可用于石见穿中主要三萜酸的定量分析。

Objective： To establish a RP-HPLC-PDA method for determination of five triterpenic acids（ pomolic acid,hawthorn acid,corosolic acid,oleanolic acid and ursolic acid） in different parts of Salvia chinensis. Method： The isocratic elution and separation was achieved on a Kromasil C18column（ 4. 6 mm × 250 mm,5 μm）,using acetonitrile-water（ 90∶ 10） as the mobile phase at a flow rate of 0. 8 m L·min^-1. The detection wavelength and column temperature were set at 205 nm and 28 ℃,respectively. Result： The calibration curves of pomolic acid,hawthorn acid,corosolic acid,oleanolic acid and ursolic acid were linear over the ranges of 0. 096 0-2. 400,0. 123 0-3. 075,0. 242 0-6. 050,0. 283 0-7. 075 and 0. 273 0-6. 825 μg（ r = 0. 999 8,0. 999 7,0. 999 9,0. 999 5,0. 999 9）,respectively. The average recoveries were 98. 43%,98. 13%,100. 6%,98. 19% and 99. 15% %,respectively,with RSD（ n = 6） being 1. 3%,0. 67%,1. 2%,0. 87% and 0. 43%. Conclusion： The proposed method is so simple and highly reproducible that it promises to be applicable for determination of major triterpenic acids in S. chinensis.