急性早幼粒细胞白血病微小残留病灶的检测

Detection of minimal residual disease in acute promyelocytic leukemia

目的探讨急性早幼粒细胞白血病(APL)患者免疫分型与微小残留病灶的特点及临床意义。方法对30例急性早幼粒细胞白血病患者采用CD45/SSC双参数散点图设门方法进行三色流式细胞术免疫分型分析,多色单克隆技术检测微小残留病灶。结果 30例急性早幼粒细胞白血病(APL)主要阳性表达为CD33、CD117、CD13、CD38、MPO和CD11B(阳性表达率分别为100%、70.00%、76.67%、53.33%、76.67%、10.0%);HLA-DR和CD34阳性表达率分别为6.67%和30.0%。结论免疫分型在细胞形态学和细胞化学染色基础上对急性早幼粒白血病诊断与分型极为重要,同时对急性早幼粒细胞白血病的免疫分型的诊断结果更为客观准确。微小残留病灶的检测对白血病患者的复发能够提前预测,对于疾病的判断和预后至关重要。

Objective To study the characteristics and clinical significance of immunophenotype and minimal residual diseases of acute promyelocytic leukemia（APL） patients. Methods The immunophenotypes of 30 acute promyelocytic leukemia patients were analyzed by using three - color flow cytometry. The minimal residual diseases were detected by multicolor monoclonal tech- nology. Results The positive rates of CD33, CDll7, CD13, CD38, MPO and CDllB were 100% , 70.00% , 76.67% , 53.33% , 76.67% , 10.0% respectively in 30 acute promyeloeytic leukemia（APL） patients. HLA - DR and CD34 can be detected in 6.67% and 30.0% patients, respectively. Conclusion Immunophenotyping is important to the diagnosis and typing of acute promyeloctic leukemiaon on the basis of cell morphology and cell chemical staining. Meanwhile, the results of immunophenotyping of acute promyelocytic leukemia are more objective and accurate. The detection of minimal residual leukemia can predict the relapse of leukemia patients in advance, which is crucial to diagnosis and prognosis of the disease.