银杏叶提取物阻断N-亚硝基化合物合成的研究

Study on EGB extract inhibiting the formation of N-nitroso compound

目的探讨银杏叶提取物阻断小鼠体内N-亚硝基化合物合成的作用,为银杏叶提取物的抗氧化能力和免疫抑制作用提供实验依据。方法灌胃给予小鼠生理盐水、亚硝酸钠、亚硝酸钠+低浓度EGb、亚硝酸钠+中浓度EGb、亚硝酸钠+高浓度EGb,所有小鼠分笼饲养,2周实验后提取各组小鼠血清和肝脏,-20℃保存,检测小鼠MDA、SOD及GSH等指标。结果 Na NO2组的平均肝重及肝/体重比均明显高于对照组(P<0.01);Low EGb、Middle EGb组的平均肝重及肝/体重比高于对照组,差异有统计学意义(P<0.05);High EGB组的平均肝重及肝/体重比略高于对照组,无统计学意义(P>0.05)。结论EGB能降低小鼠血清MDA含量,同时能提高血清SOD和GSH活性,提高机体抗氧化能力。

Objective To investigate the inhibiting effect of EGB extract on composing of N-nitroso compound in mice , and provide an experimental basis for the EGB extract ＆#39;s antioxidant ability and immunesuppression .Methods Administrated mice with normal saline , NaNO2, NaNO2 ＋low concentration EGB, NaNO2＋middle concentration EGB , NaNO2＋high concentration EGB .All mice were housed separately in standard solid state .After 2 weeks,we detected the concentration of MDA , SOD and GSH in serum and liver tissue.Results The liver weight and liver/body weight ratio in NaNO2administrated groups were significantly higher than the control group （P〈0.01）.The liver weight and liver/body weight ratioof low concentration EGB and middle concentration EGBgroup were significantly higher than the control group （P〈0.05）.The liver weight and liver/body weight ratioof high concentration EGBgroup were higher than the control group , but present no statistically significant（P〉0.05）.Conclusions EGB can reduce the concentration of MDA in serum , increase the activity of SOD and GSH , and improve antioxidant capacity .