CPPs-BDI-1-载蟾酥白蛋白纳米微球三联体杀伤EJ人膀胱癌细胞实验研究

Experimental research of CPPs-BDI-1-Bufalin-Loaded albumin nanoparticle triad killing EJ human bladder cancer cells

目的探讨CPPs-BDI-1-载蟾酥白蛋白纳米微球三联体杀伤EJ人膀胱癌细胞的实验研究。方法采用异型双功能连接剂SPDP耦联穿膜肽增强型绿色荧光蛋白(TAT-EGFP)、抗人膀胱癌单克隆抗体(BDI-1)、载蟾酥白蛋白纳米微球(Bufalin-NS),构建载蟾酥白蛋白纳米微球三联体(CPPs-BDI-1-Bufalin-NS)。取CPPs-BDI-1-Bufalin-NS三联体实验组和蟾酥对照组,终浓度分别为1×10^(-8)mol/L、1×10^(-6)mol/L,通过MTT法检测培养12小时、24小时、36小时、48小时的EJ人膀胱癌细胞存活率,检测CPPs-BDI-1-Bufalin-NS三联体对EJ人膀胱癌细胞的抑制作用。结果 CPPs-BDI-1-Bufalin-NS三联体实验组杀伤人膀胱癌细胞作用明显强于蟾酥对照组。结论 CPPs-BDI-1-Bufalin-NS三联对人膀胱癌细胞有明显的杀伤效果,为后续动物实验奠定基础。

Objective To explore the experimental research of CPPs–BDI-1–bufalin–Loaded albumin nanopar-ticle triad killing EJ human bladder cancer cells. Method Use SPDP decoupling league to build nanometer micro-spheres triplet CPPs-BDI-Bufalin-NS with the preparation of TAT-EGFP, BDI-1-NS, Bufalin-NS. Take CPPs-BDI-1-Bufalin-NS triplets as experimental group and bufalin control group, with the final concentration of 1×10-8mol/L and 1×10-6mol/L, by MTT method detecting the survival rate of EJ human bladder cancer cells after 12h, 24h, 36h, 48h, which detect the inhibition effect of CPPs-BDI-1-Bufalin-NS triplets killing human bladder cancer cells. Result The inhibition effect CPPs-BDI-1-Bufalin-NS triplet killing EJ human bladder cancer cells was obviously stronger than the bufalin control group. Conclusion It was a obvious killing effect that CPPs-BDI-1-Bufalin-NS trip-lets killed human bladder cancer cells and laied the foundation for subsequent animal experiment.