摘要
目的:探讨血清胸苷激酶(TK1)水平在健康体检人员中不同性别、不同年龄中的差异,以及在健康体检人员中血清胸苷激酶(TK1)的阳性率及在肿瘤早期筛查中的作用和对于进展期癌前病变恶性风险进程的跟踪研究。方法:选取1200例健康体检人员根据性别和年龄分为男性年龄(20-39岁)组、男性年龄(40-80)组;女性年龄(20-39岁)组、女性年龄(40-80)组;;应用免疫印迹-增强化学发光法检测血清胸苷激酶(TK1)水平,采用χ2及Fisher确切概率法分析。结果:TK1检测灵敏度为0.2 pmol/L-(2)男性634例,平均年龄48.5岁(22-80岁),TK1水平为0.15-54.90(1.164士2.092) pmol/L,阳性(〉=2.0)30例,阳性率4.47%;女性586例,平均年龄46.0岁(21-77岁),TK1水平为0.11-14.53(1.198±1.053)pmol/L,阳性(〉=2.0)46例,阳性率7.81%;男女性别比较P =0.069,无显著差异;(3)男性年龄(20-39岁)组TK1水平(1.023±0.661) pmol/L.男性年龄(40-80)组TKl水平((1.194±2.609) pmol/L,两组比较P=0.11,无显著差异;女性年龄(20-39岁)组TKI水平(1.19±0.825) pmol/L,女性年龄(40-80) TK1水平(1.21±1.125) pmol/ L,两组比较P=0.27.无显著差异。结论:(1) TKI水平在健康体检人员中含量差别甚远,但不同性别、不同年龄中的差异经统计学处理均无显著差异(2)TK1水平在健康体检人员中阳性率较高,表明影响TK 1的因素较多必需排除一些生理状态等因素引起的TKI水平升高(3)血清TK1表达具有很高的增殖特异性和灵敏度,因此,在肿瘤早期筛查中有重要的应用价值。
Objective:To investigate the serum thymidine kinase (TK1) levels of different sex, age differences, and serum thymidine kinase (TK1) positive rate and its role in healthy volunteers in early tumor screening in healthy volunteers and in follow-up study of the progress of the risk of precancerous lesions and malignant processes.Methods:The results TK1 detection sensitivity is 0.2 pmol/L- (2) male 634 cases, with an average age of 48.5 years (22-80 years old), TK1 level of 0.15-54.90 (1.164 ± 2.092) pmol / L, positive (〉 = 2.0n = 30), the positive rate of 4.47%; female 586 cases, with an average age of 46.0 years (21-77 years old), TK1 level of 0.11-14.53 (1.198 ± 1.053) pmol / L, positive (〉 = 2.0) 46 cases, the positive rate 7.81%; gender comparisonP=0.069, no significant difference;. (3) Male (20-39 years old) group TK1 levels (1.023 ± 0.661) pmol / L. Male Age (40-80) group TKl level ( (1.194 ± 2.609) pmol / L, the two groupsP=0.11, no significant differences; women age (20-39 years old) group TKI levels (1.19±0.825) pmol / L, Female (40-80) TK1 levels (1.21±1.125) pmol / L, the two groupsP=0.27. was no significant difference in the.Results:(1) TKI level in healthy volunteers in far content varies, but a different gender, age differences in the statistical processing of the data There was no significant difference (2) TK1 levels higher in healthy volunteers positive rate, indicating that the impact of a higher TK factors necessary to exclude some of the physiological state of TKI level rise caused by other factors (3) serum TK1 expression with high proliferative specificity and sensitivity, therefore, has important applications in early cancer screening.
出处
《医学检验与临床》
2014年第5期16-18,共3页
Medical Laboratory Science and Clinics