IL-4对小鼠小胶质细胞ICAM-1表达的影响

Effects of IL-4 on the expression of ICAM-1 in microglia cells

目的探讨白细胞介素-4(IL-4)对小鼠小胶质细胞炎症因子细胞间粘附分子-1(ICAM-1)表达的影响。方法用IL-4与小鼠小胶质细胞共同孵育,观察不同处理组细胞形态的变化。ELISA法检测IL-4孵育小胶质细胞培养上清中ICAM-1的浓度,同时采用RT-PCR技术检测细胞ICAM-1mRNA水平。结果 IL-4与小胶质细胞共同孵育后细胞形态随孵育时间发生变化,贴壁细胞逐渐减少,IL-4减少小胶质细胞分泌ICAM-1并下调mRNA的表达水平。结论 IL-4可能通过抑制ICAM-1的产生及分泌而参与小胶质的细胞迁移过程,从而在中枢神经系统中发挥神经保护作用。

Objective To explore the impact of IL- 4 on the expression of intercellular adhesion molecule- 1（ ICAM- 1） in murine microglia cells. Methods Murine microglia cells were cultured in the presence of IL- 4. After co- incubation,morphological changes of the cell were observed through a microscope. The level of ICAM- 1 in the culture supernatant was measured by enzyme- linked immunosorbent assay（ ELISA）,and the mRNA level of ICAM- 1 in microglia cells was detected by RT- PCR. Results The cell morphology of microglia cells after incubation with IL- 4 changed over time,and adherent cells gradually reduced. Both the ICAM- 1 level in the culture supernatant and ICAM-1 mRNA expression in microglia cells were decreased by IL- 4 co- incubation. Conclusion IL- 4 might promote microglia cells migration by inhibiting the expression and secretion of ICAM- 1 and thereby exert neuroprotective function.