福州旗山野生蕉(Musa spp.,AB Group)试管苗Mn-SOD基因克隆及生物信息学分析

Cloning and Bioinformatics Analysis of Mn-SOD Gene from the Plantlets of a Wild Banana in Qishan Mountain of Fuzhou(Musa spp., AB Group)

以旗山野生蕉(Musa spp.,AB group)试管苗幼嫩叶片为材料,利用RT-PCR结合RACE技术克隆获得野生蕉试管苗Mn-SOD基因c DNA序列。结果表明:旗山野生蕉Mn-SOD的c DNA全长序列共831 bp,其中5′UTR为137 bp,3′UTR为151 bp,3′端含有17个poly(A)尾。开放阅读框(ORF)共有543个碱基组成,编码181个氨基酸。蛋白理化性质预测结果显示:Mn-SOD蛋白分子量为20 108.8 u,等电点7.92,属于碱性蛋白。

The young leaves of in vitro plantlets of a wild banan （Musa spp., AB group）grown in Qishan Mountain of Fuzhou were used as the materials to clone Mn-SOD gene by RT-PCR and RACE technology. The full-length cDNA sequence was 831 bp, containing 137 bp 5＇UTR, 543 bp open reading frame, encoding 181 amino acids, 151 bp 3＇UTR, and 3＇-end involved 17 poly（A）tails. The prediction of protein＇s physical and chemical properties showed it belonged to a basic protein with 20 108.8 u and pi7.92.