酶催化酸解棉籽油分提产物合成结构脂质研究

Enzymatic Acidolysis Synthesis of Structured Lipids from Products of Cottonseed Oil

以棉籽油分提产物和辛酸为原料,Sn1,3-位定向固定化脂肪酶为催化剂,在无溶剂体系中催化酸解棉籽油分提产物合成一类具有供能低、易消化、熔点低等优点的功能性结构脂质。对3种不同碱液进行脱酸能力比较,结果表明KOH-乙醇水溶液脱酸效果最好;对3种不同来源的脂肪酶脱酸能力进行筛选,结果表明Lipozyme RM IM脂肪酶催化活性高;在单因素试验基础上,以辛酸含量为响应值,采用响应面试验确定最优工艺条件为:反应温度68℃,反应时间7.88 h,酶添加量11.8%,底物比2.98∶1。在最佳反应条件下,产物中辛酸质量分数达35.25%。

The research took cottonseed oil products and caprylic acid as raw materials, Snl ,3 - directional im- mobilized lipase as catalysted, which catalyzes acidolysis reaction of cottonseed oil products in free - solvent to pro- duce structured lipid of low energy, easy to digest and low melting point. Three kinds of Lye were compared on their ability of acid removal and the results indicated that the KOH - ethanol water had the best acid removal ability. Three different sources of lipases were screened for their ability to catalyze acidolysis and the results indicated that the Li- pozyme RM IM had the best catalysis ability. Based on the single factor experiment, the caprylic acid content as the response value, the optimal process conditions were as follows ： reaction temperature of 68 ℃, reaction time of 7.88 h, enzyme dosage of 11.8% , molar ratio of caprylic acid to cottonseed oil fraction of 2.98：1 using response surface method. Under the optimal condtions, the eaprylic acid mass fractions in the finished product were 35.25%.