Lowry法测定人凝血因子Ⅷ蛋白含量

Determination of protein assay of human coagulation factor Ⅷ by Lowry method

目的:对Lowry法应用于人凝血因子Ⅷ蛋白含量测定中的可行性进行研究。方法:考察S公司人凝血因子Ⅷ原液及成品,比较凯氏定氮法与Lowry法对蛋白测定结果产生的差异,及不同浓度保护剂对Lowry法的影响。结果:甘露醇对蛋白含量测定有明显正干扰,甘氨酸有负干扰;对样品进行预稀释,辅料在低浓度范围内,当甘露醇浓度为0.1%和甘氨酸浓度为0.3%时,可减少辅料的干扰作用。结论:适当稀释样品可减少保护剂的干扰,用Lowry法测定人凝血因子Ⅷ蛋白含量,简单快速准确可行。

Objective ：To determine the protein content in human coagulation factor Ⅷ（FⅧ）by Lowry method. Methods：Lowry method can he used to investigate 3 samples from S company compared with kjeldahl method at the present of different interfering substances. Results：The data showed that mannitol for determination of protein content had significant positive interference, however, glycine had a contrary tendency. The present of 0.1% mannitol and 0.3% glycine showed less interference to the determination result by Lowry method at concentrations of pre-dilute samples. Conclusions： Lowry method can be used to determine protein content of human coagulation factor Ⅷ at certain low concentration solution, which is simple, rapid and feasible.