外周血特异性T淋巴细胞IFN-γ释放试验在辅助AIDS病人合并活动性TB感染诊断中的作用

The diagnostic value of peripheral blood specific T-lymphocyte IFN-γrelease assay in active tuberculosis infection in AIDS patients

目的对怀疑合并结核(TB)的艾滋病(AIDS)病人的特异性外周血T淋巴细胞γ干扰素(IFN-γ)的检测结果,与临床及实验室诊断结果进行比较,探讨该方法在AIDS合并活动性TB中的辅助诊断价值。方法收集怀疑合并TB的AIDS病人的临床资料,对其TB患病情况进行综合分析。同时运用特异性外周血T淋巴细胞IFN-γ释放试验,对怀疑合并TB的AIDS病人进行检测。结果 22例AIDS病人中,确诊合并活动性TB的共9例(临床确诊TB 6例,实验室确诊TB 3例),酶联免疫斑点法(Elispot)阳性6例,其中临床确诊的活动性TB病人5例。暂未诊断活动性TB的11例病人中,7例Elispot阳性。结论特异性外周血T淋巴细胞IFN-γ释放试验,在AIDS合并活动性TB的诊断中具有重要价值。

Objective To compare Elispot-IFN-γ results with clinical and laboratory diagnosis in suspected tu- berculosis （TB）, and to appraise the diagnostic value of Elispot-IFN-γ assay in active TB infection in AIDS patients. Methods Clinical data of AIDS patients suspected TB infection were collected and analyzed. Elispot-IFN-γ assay was performed. The assay results and clinical diagnosis were compared. Results Of 22 AIDS patients suspected TB infection, 9 were definitely diagnosed TB infection （six were clinically diagnosed and three were laboratorily diag- nosed）. Six subjects had positive Elispot results among which 5 were active. Of 11 inactive TB patients, 7 were Elispot positive. Conclusions It is clear that peripheral blood specific T lymphocyte IFN-γ release assay is valuable in diagnosis of active TB in AIDS patients.