大连市HIV-1亚型分布及流行特征分析

Study on subtype distribution and epidemiological characteristics of HIV-1 infection in Dalian City

目的了解大连市近年新确证艾滋病病毒(HIV)感染者HIV-1的基因亚型分布及其流行特征。方法对144份全血标本分离血浆,提取核糖核酸,巢式聚合酸链反应扩增及序列测定,对测得的序列进行生物信息学分析,确定基因亚型,分析流行特征。结果 144份全血标本中,得到103例HIV感染者的有效基因序列,包括CRF01-AE、B(B’)、C、CRF-BC亚型及未确定重组型。其中CRF01-AE重组型占62.14%(64/103),B(B’)占25.24%(26/103),C亚型占1.94%(2/103),BC重组型占9.71%(10/103),未确定重组型占0.97%(1/103)。系统进化树显示,大连毒株与东北、北京等地区的毒株亲缘关系较近,存在交叉传播。结论大连市HIV感染者中,CRF01_AE为主要流行亚型,性途径尤其是男男同性传播是各亚型主要传播途径,与全国范围分子流行病调查结果有统计学差异。

Objective To study subtype distribution and epidemiological characteristics of HIV-1strains prevalent in Dalian in recent years.Methods Viral RNA was extracted from plasma samples,and HIV-1genes（env and gag）were amplified by RT and nested-PCR using specific primer pairs and sequenced directly.The acquired sequences were compared with international subtypes references and their phylogenetic trees were analyzed to determine the subtype.Results Env gene fragments of HIV-1were successfully amplified in 103 samples,of which CRF01_AE accounted for 62.14%（64/103）,B（B＇）25.24%（26/103）,C1.94%（2/103）,CRF_BC 9.71%（10/103）and indeterminate CRF 9.71%（10/103）.The phylogenetic tree showed that Dalian strains were closely related to strains in Northeast China and Beijing and there was a cross during HIV spread.Conclusion CRF01_AE is the major subtype and homosexual transmission is the major route of transmission of the subtypes in Dalian.There are significant differences from the results of a nationwide survey of molecular epidemiolog