摘要
免疫组化染色主要原理是用标记的抗体对细胞或组织内的相应的抗原进行定性,定位或定量检测。经过组织化学的呈色反应而呈现醒目的阳性色彩,用显微镜,荧光显微镜或电子显微镜观察。凡是能作为抗原、半抗原的物质,都可用相应的特异性抗体在组织、细胞内应用免疫组化方法可准确的定位。熟练掌握一种免疫组化技术操作方法步骤,则其它的方法和步骤也基本上掌握了。
The principle of immunohistochemical staining is to utilize marked antibodies to qualitative,position or quantitative corresponding antigen in cells or tissues.With histochemitry,color reaction shows striking positive colors,which can be observed by microscope,fluorescence microscope or an electron microscope.Any substance that can be used as antigens or haptens is able to be precisely positioned within tissues and cells by immunohistochemical method with the aid of corresponding specific antibodies.Having a good mastery of immunohistochemical technique operation method and procedure,the other methods and procedures are acquired basically.
出处
《中国卫生标准管理》
2014年第21期132-133,共2页
China Health Standard Management
关键词
免疫组化染色
基本方法
Immunohistochemical staining
Basic method
