红景天苷对百草枯中毒大鼠肺组织TGF-β1表达的影响

Effect of Salidroside on the Expression of TGF-β1 in Lung Tissue of Acute Paraquat Poisoned Rats

目的研究红景天苷(salidroside,SDS)对百草枯(paraquat,PQ)中毒大鼠肺组织TGF-β1表达的影响。方法将130只SD大鼠随机分为正常对照组、模型对照组、SDS治疗组,后2组又分为1,3,7,14,21,28 d 6个亚组,每亚组10只。模型对照组和SDS治疗组采用一次性灌胃百草枯溶液(20 mg·m L?1)制备急性PQ中毒肺损伤模型,染毒1 h后分别给予相同体积的生理盐水、SDS(10 mg·m L?1)腹腔注射。各组在相应时间点取材,采用免疫组化、RT-PCR测定肺组织中转化生长因子β1(TGF-β1)及其m RNA的表达情况。结果免疫组化结果显示,与正常对照组比较,模型对照组、SDS治疗组肺组织TGF-β1表达显著增加(P<0.05),在第14天达到最大值。与模型对照组各时间点比较,SDS治疗组中TGF-β1的表达量低,两者差异有统计学意义(P<0.05)。RT-PCR结果显示模型组与SDS治疗组染毒后第1天大鼠肺组织TGF-β1m RNA水平开始增加,在第14天达到最高值,第28天基因表达恢复正常。第3天至第28天SDS治疗组TGF-β1 m RNA表达量比模型对照组低,两者比较差异有统计学意义(P<0.05)。结论 TGF-β1参与了PQ中毒大鼠肺纤维化病理生理过程,SDS能抑制TGF-β1的表达,减轻PQ中毒大鼠肺损伤。

OBJECTIVE To investigate the effect of salidroside（SDS） on the expression of transforming growth factor-β1（TGF-β1） in lung tissue of acute paraquat（PQ） poisoned rats. METHODS All of 130 SD rats were randomly divided into normal control group, model group and SDS group, the latter two groups were then divided into 1, 3, 7, 14, 21, 28 d group. The rats of model group and SDS group were given a single dose of PQ（20 mg·m L^-1） intraperitoneally to induce lung injury, and after 1 h, the same volume of saline and SDS（10 mg·kg^-1） was intragastricly administrated respectively per 12 h. The rats were executed in each time point, and use methods of immune histochemistry and real-time PCR to observe the TGF-β1 expression of both protein and m RNA level. RESULTS The expression of TGF-β1 in model group and SDS group were significantly increased compared with normal control group, and peaked at 7 d after injectd PQ intraperitoneally. Compared with model group, the expression of TGF-β1 was lower in different time points（P〈0.05）. The level of TGF-β1 m RNA was increased at the first day, peaked at the 14 th day and then returned to normal at the 28 th day. Compared with model group, the level TGF-β1 m RNA was lower in SDS group from the 3th day to 28 th day（P〈0.05）. CONCLUSION TGF-β1 is involved in the pathological and physiological process of pulmonary fibrosis. SDS can inhibit its expression, and alleviate the lung injury induced by PQ.