心脑宁片对大鼠心肌缺血模型ATP酶、LDH及CK活性的影响

Effect of Xinnaoning Tablets on the ATP Enzyme, LDH and CK Activity of the Rat Myocardial Ischemia Model

目的研究心脑宁片对大鼠心肌缺血模型ATP酶、LDH及CK活性的影响。方法选取体质量为230-250 g大鼠70只,♀♂各半,分为7组,其中第1组为空白对照组,其余6组造心肌缺血模型。6组造模大鼠分别灌服高、中、低剂量的心脑宁片混悬液、硝苯地平混悬液、脑心通胶囊混悬液和同体积的0.5%羧甲基纤维素钠。观察心电组织中ATP酶活力,检测血清中LDH、CK活性,并测定心电图变化。结果与模型组比较,高、中、低剂量心脑宁片组、脑心通胶囊组和硝苯地平片组均可升高心肌组织匀浆中Na^＋-K^＋-ATPase活力（P〈0.01）;中、低剂量心脑宁片组可升高心肌组织匀浆中Mg2＋-ATPase活力（P〈0.01或P〈0.05）;低剂量心脑宁片组可升高心肌组织匀浆中Ca^2＋-ATPase活力（P〈0.01）。与模型组比较,高、中、低剂量心脑宁片组、脑心通胶囊组和硝苯地平片组均可不同程度地降低血清中LDH活力（P〈0.01）;高、中、低剂量心脑宁片组、脑心通胶囊组和硝苯地平片组均可降低血清中CK活力（P〈0.01或P〈0.05）,高、中、低剂量心脑宁片组可显著减少心电图ST段的升高。结论心脑宁片组能有效升高ATP酶活力,降低LDH、CK活力,减少心电图ST段的升高。

OBJECTIVE To study the effect of Xinnaoning tablets on the ATP enzyme, LDH and CK activity of rat myocardial ischemia model. METHODS The seventy rats of half male and half female were divided into seven groups. The first group was the control group, and the other six groups were made the model of myocardial ischemia. Six groups of rats were fed with different doses of Xinnaoning tablets suspension, nifedipine suspension, Nao-xin-tong capsule suspension, nifedipine suspension, Nao-xin-tong capsule suspension and the same volume of 0.5% CMC. The activity of ATP enzyme LDH and CK were observed, and the diog electrocardiogram was draw as well. RESULTS Compared with model group, three doses of Xinnaoning tablets groups, Nao-xin-tong capsule group and nifedipine tablets group could increase the Na^＋-K^＋-ATPase activity of myocardial tissue homogenate（P〈0.01）; medium and low dose of Xinnaoning tablets group could increase the Mg2＋-ATPase activity of myocardial tissue homogenate（P〈0.01 or P〈0.05）; low dose of Xinnaoning tablets group could increase the activity of Ca^2＋-ATPase in myocardial tissue homogenate（P〈0.01）. Compared with the model group, three doses of Xinnaoning tablets groups, Nao-xin-tong capsule group and nifedipine tablets group could significantly reduce the activity of serum LDH（P〈0.01）; High, medium and low doses of Xinnaoning tablets groups, Nao-xin-tong capsule group and nifedipine tablets group could decrease the activity of CK in serum（P〈0.01 or P〈0.05）. Xinnaoning groups could reduce the elevation of ST segment. CONCLUSION Xinnaoning tablets group can significantly improve the activity of ATPase, reduce the activity of LDH and CK.