摘要
采用循环伏安法和紫外光谱法研究了羧甲基壳聚糖-Cr(III)配合物与鲱鱼精DNA之间的作用方式。结果发现:(1)羧甲基壳聚糖-Cr(III)的存在导致Fe(CN)3-6/4-探针分子氧化还原峰电流下降,式量电位正移,显示羧甲基壳聚糖-Cr(III)和Fe(CN)3-6/4-与DNA之间存在竞争性作用,说明羧甲基壳聚糖-Cr(III)通过插入方式与DNA相互作用的;(2)中性红的加入导致羧甲基壳聚糖-Cr(III)/DNA体系的特征吸收峰发生增色效应、峰位红移并伴有等吸收点出现,这进一步表明羧甲基壳聚糖-Cr(III)是以插入方式与鲱鱼精DNA发生了相互作用,导致DNA分子的构象变化,并且CMC-Cr与DNA的结合比n(CMC-Cr):n(DNA)为3∶1。
The interaction between carboxymethylehitosan-Cr （Ⅲ） Complex （CMC-Cr） and herring sperm DNA were investigated by cyclic voltammetry and UV-Vis spectra. The results indicated：Firstly,the peak current of probe molecule Fe （CN）3-/6 at Au/DNA electrodes obviously decreased and the peak potential shifted positively because of the exist- ences of CMC-Cr,which showed that there was a competitive effect between CMC-Cr and Fe （CN）3-/6 /4 with herring sperm DNA,and revealed that a binding mode intercalation was interacted between CMC-Cr and herring sperm DNA. Secondly, the absorption intensity of CMC-Cr/DNA system increased with the adding of neutral red （NR）, the maximum absorption peak had a red shifted and appeared equal absorption point, which further illustrated that a binding mode intercalation was interacted between CMC-Cr and herring sperm DNA, thus led to the change of DNA conformation. The combining ratio of n（CMC-Cr） and n（DNA） was 3： 1.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2014年第11期1757-1760,共4页
Natural Product Research and Development
基金
天水师范学院"青蓝"人才工程和中青年科研项目(TSA1305
TSA1307)
