壳聚糖载黄芪多糖纳米粒调节糖尿病小鼠免疫功能

Astragalus Polysaccharide Nanoparticle Adjusts Immune Function of Diabetes Mellitus Mice

研究低分散度壳聚糖载黄芪多糖纳米粒(LCA)对糖尿病(DM)小鼠免疫功能的影响。注射链脲佐菌素与环磷酰胺混合试剂建立DM合并免疫力低下小鼠模型,酶法制备低分散度壳聚糖,离子交联法制备低分散度壳聚糖纳米粒,超声包埋黄芪多糖制备药物对昆明小鼠灌胃,每天1次,连续30 d。ELISA法检测小鼠血清Ig M、Ig G与INF-γ的含量,碳粒廓清法测定非特异性免疫功能,耳肿胀法检测迟发型变态反应,MTT法检测脾淋巴细胞增殖率以反映细胞免疫功能。结果显示灌胃350 mg/(kg·d)LCA显著提高血清Ig M、Ig G的分泌,显著降低INF-γ表达量,增强碳粒廓清率,提高小鼠迟发型变态反应(DTH),改善脾淋巴细胞增殖反应。适当剂量的低分散度壳聚糖载黄芪多糖纳米粒能提高DM小鼠体液免疫、非特异性免疫及细胞免疫功能,且效果优于纯黄芪多糖。

The aim of this study was to investigate the effects of astragalus polysaceharide （APS） nanoparticle （using chitosan as carrier） with low dispersion on immune function of diabetes mellitus （DM） mice. The DM low immunity mice model was developed by injecting cephalospofins and cyelophesphamide. The low dispersion chitosan was prepared by enzymatic method. The low dispersion chitasan APS nanoparticle was then prepared by ultrasonic embedding. The prepared sample was garaged to the developed mice model, one time a day and for 30 d. The levels of IgM, IgG and INF-γ of DM mice serum were determined by ELISA method. The nonspecific immune function of DM mice was determined by carbon clearance method. The delayed type hypersensitivity （DTH） was determined by ear swelling method and the spleen lymphocyte proliferation rate was determined by MTI＇ assay and was used to reflect the cellular immune function. The experimental results showed that the 350 rag/（ kg ~ d） LCA group significantly increased the secretion of serum IgM and IgG, significantly reduced the INF-γ expression, enhanced the carbon clearance rate, improved the mice DTH, improved spleen lymphocyte proliferation reaction. It indicated that appropriate doses of low dispersion ehitosan APS nanoparticle can improve DM mice humoral immunity, nonspeeific immunity and cellular immunity function, can reduce the incidence of DM complicated with infection. The effect of low dispersion chitosan APS nanoparticle was borer than that of pure APS.