阿纳其根脂肪酸成分分析及其对蛋白酪氨酸磷酸酯酶1B抑制作用研究

Study on Component and PTP1B Inhibition Activity of Fatty Acids of Anacyclus pyrethrum(L) DC

利用三种不同溶剂(正己烷、乙醚、氯仿)经索氏提取法提取阿纳其根中的油脂成分,经甲酯化后,用气相色谱质谱法(GC-MS)对脂肪酸组成进行了分析和鉴定。试验结果显示,正己烷提取油脂中共鉴定出了8种脂肪酸,其中亚油酸为36.341%、棕榈酸为34.268%、油酸为19.656%;乙醚提取油脂中共鉴定出了12种脂肪酸。其中亚油酸为34.102%、棕榈酸为32.916%、油酸为20.000%、亚麻酸为5.329%;氯仿提取油脂中共鉴定出了5种脂肪酸。其中亚油酸为42.427%、棕榈酸为40.952%、油酸为11.250%。对阿纳其根脂肪酸进行蛋白酪氨酸磷酸酯酶1B(PTP1B)的抑制作用研究,结果表明脂肪酸对PTP1B有较强的抑制作用,其IC50分别为5.27±0.40、4.71±0.08、7.01±1.98μg/m L。该研究为深入研究阿纳其根的药理作用提供了科学依据。

Fats and oil components were extracted by Soxhlet extraction from Anacyclus pyrethrum （L） DC using three different organic solvent （n-hexane, diethyl ether, chloroform）. The chemical constituents of fatty acids in different ex- tracts were identified and semi-quantified by gas chromatography-mass spectrometry （GC-MS）. The results indicated that 8 components, mainly including linoleic acid （36. 341%）, hexadecanoic acid （34. 268%） and oleic acid （19. 656% ）, were detected in the n-hexane extract; 12 components, mainly including linoleie acid （34. 102% ）, hexa- decanoie acid （32.916%） ,oleic acid （20. 000% ） and α-linolenie acid （5. 329%.） ,were detected in the diethyl ether extract;5 components, mainly including linoleic acid （42. 427% ）, hexadecanoic acid （40. 952% ） and oleic acid （11. 250% ） ,were detected in chloroform extract. In addition,the fatty acid extracts showed strong inhil^iting effect on PTP1 B, and their ICso values were 5.27±0.40,4.71±0.08,7.01± 1.98μg/mL respectively. The results provided the basis for further study of Anacyelus pyrethrum （L） DC.