丁岙杨梅挥发油成分及抗肿瘤活性的研究

Volatile Constituents from Myricarubra cv.Ding Ao Orient Pearl and Its Antitumor Activity

采用水蒸气蒸馏法提取丁岙杨梅果实挥发油,通过气相色谱-质谱联用法(GC-MS)分析其化学成分,同时使用MTT法检测其对肿瘤细胞的体外增殖的抑制活性。从丁岙杨梅果实挥发油中分离得到33个峰,鉴定出了其中的27个化合物,已鉴定的化合物占总组分的92.37%,其主要成分为:石竹烯(20.80%)、邻苯二甲酸己烷-3-醇异丁醇酯(9.73%)、棕榈酸(26.55%)、2,2'-亚甲基双-(4-甲基-6-叔丁基苯酚)(6.55%)、邻苯二甲酸二异辛酯(4.23%)、β-谷甾醇(3.23%)等。丁岙杨梅果实挥发油表现出了较好的抗肿瘤活性,浓度为200μg/m L时,对肿瘤细胞MGC-803、MCF-7和A549体外增殖的抑制率分别为57.3%、42.0%和42.4%。丁岙杨梅果实挥发油化学成分复杂,含有大量的有机酸、酯、萜、酚、甾体、烷烃等,同时也对肿瘤细胞的体外增殖表现了较好的抑制作用。

To study the chemical compositions of volatile oil from Myricarubra cv. DingAo Orient Pearl and its anti-tumor activity. The volatile oil from M. rubra was extracted by steam distillation, and was identified by gas chromatography-mass spectrometry （GC-MS）. The antitumgr effect of the oil was evaluated by MTr assay. Twenty-seven compounds were i- dentified,accounted for 92.37% of the extract. The major components were caryophyllene （20.80%）, phthalic acid, hex-3-yl isobutyl ester （ 9.73% ）, n-hexadecanoic acid （26.55%）, phenol ,2,2＇-methylenebis [ 6-（ 1,1 -dlmethylethyl） - g-methyl （6.55%）, diisooctyl phthalate （4.23 % ） and/3-sitosterol （3.23%）. The oil showed significant antitumor ac- tivities against MGC-803, MCF-7, and A549 cells （tumor growth inhibitory rates were 57.3%, 42.0%, and 42.4% at 200 μg/mL, rospectively ）.