摘要
通过紫外线光谱扫描、清除自由基试验和光溶血试验等方法对不同生长期、不同部位的汉麻提取物进行了抗紫外评价和比较,优选出的提取部位为:汉麻(160 d)叶的95%乙醇提取物,并进一步确定其最佳提取工艺条件为:料液比1∶20,提取温度75℃,提取时间2 h,提取1次。汉麻叶提取物的主要成分为黄酮类物质(0.571 g/g)。在安全性评价中,红细胞溶血试验和鸡胚绒毛尿囊膜试验的结果均表明该汉麻叶提取物无明显刺激性。此外,该提取物还具有良好的的p H、冷、热和光稳定性,可作为一种抗紫外的植物功效原料应用于防晒化妆品的开发。
The anti-UV efficacy and safety of extracts of Cannabis sativa L. （China-hemp） of different growth stages and/or different parts were evaluated by means of ultraviolet spectral scannlng,free radical scavenging tests and light he- molysis experiments. The screened out extract with the highest anti-UV efficacy was the 95% ethanol extract of hemp leaves （160 days）. The optimized extraction parameters were determined as follows：solid-liquid ratio li20, extraction temperature 75℃, extraction time 2 h, and extract for one time. The main component of the extract was flavunoids （0. 571 g/g）. In the safety evaluation,the results of red blood cell hemolysis test and chorioallantioc membrane experiment showed that the hemp leaf extract was not irrltative. In addltian,the hemp extract also had a good stability in solution with changing pH, cold, heat and light. The 95% ethanol extract of hemp leaves （ 160 days） had the potential to be used as a functional plant additive for anti-UV cosmetics.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2014年第11期1872-1878,共7页
Natural Product Research and Development
基金
北京市优秀人才培养资助项目(20061B0500300142)
关键词
汉麻
抗紫外功效
清除自由基
光溶血实验
安全性评价
China-hemp
ultraviolet
free radical-scavenging
light hemolysis test
safety evaluation
