野生蓝莓提取物对四种致病菌的抑制作用

Antibacterial Effects of Chinese Wild Blueberry Extracts on Four Pathogenic Bacteria

本研究选用中国野生蓝莓,研究了其提取物对铜绿假单胞菌、单增李斯特菌、金黄色葡萄球菌与副溶血性弧菌等四种致病菌的抑制作用,分别采用试管二倍稀释法和平板涂布法测定了野生蓝莓提取物的最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。结果表明,野生蓝莓提取物对四种致病菌均有一定的抑制作用,对铜绿假单胞菌、单增李斯特菌、金黄色葡萄球菌与副溶血性弧菌的MIC分别为62.5、250、500 mg/m L与31.25 mg/m L,MBC分别为250、250、500与125 mg/m L。本研究首次报道了中国野生蓝莓对这四种致病菌的抑制作用,为中国野生蓝莓的开发和利用提供了依据。

The antibacterial effects of Chinese wild blueberries extracts on 4 pathogenic bacteria,Pseudomonoa aeruginosa, Listeria monocogenes,Staphylococcus aureus and Vibrio parahaemolyticus, were investigated. The testing tube double dilution method and the spread plate method were used to determine minimal inhibitory concentration （MIC） and mini- mum bactericidal concentration （MBC）. The results showed that Chinese wild blueberry extracts had antibacterial effects on four investigated pathogenic bacteria. The MIC of wild blueberry extracts against P. aeruginosa, L monocytogenes,S. aureus and K parahaemolyt/cus were 62.5,250,500 mg/mL and 31.25 mg/mL, respectively. The MBC of wild blueberry extracts against P. aeruginosa, L. monocytogenes , S. aureus , and V. parahaemolyticus were 250,250,500 mg/mL and 125mg/mL,respeetively. This was the first study that chinese wild blueberry has antibacteridal effects the antibacterial effects of Chinese on the four pathogenic bacteria. The results will benefit future application of Chinese wild blueberry.