复方利血平对小鼠肠道菌群的影响观察

Effect of compound reserpine on intestinal flora in mice

目的:观察复方利血平对正常小鼠肠道菌群的影响,为复方利血平的进一步研究和临床合理用药提供实验基础。方法:给小鼠灌服高、中剂量的复方利血平,采用粪便涂片镜检和选择性培养的方法观察复方利血平对小鼠肠道菌群的影响。结果:与空白组相比,正常小鼠灌服高、中剂量的复方利血平3 d即出现肠道菌群紊乱,但各菌落数量无显著性差异,给药6 d时复方利血平高剂量组小鼠粪便中肠球菌的数量显著增加(P<0.05),给药9 d时复方利血平高、中剂量组小鼠粪便中肠球菌的数量均显著增加(P均<0.05)。结论:长期服用复方利血平高、中剂量可导致肠道菌群紊乱,临床使用该药物时应注意患者相关的不良反应。

Objective ： To investigate the effect of compound reserpine on normal intestinal flora of mice in order to provide reference for their safe use in clinic. Methods： Compound reserpine （81.30 or 24.45 μg·kg-1） was orally administered to normal mice twice a day for 9 days. The changes in intestinal flora were observed by Gram-staining microscopic examination and a selective culture method. Results： Compared with control group, the disturbance of intestinal flora in normal mice was caused by both doses three days after administration, but the quanti- ty amounts of 4 bacterial kinds were similar. The number of enterococcus was significantly increased at 6 days after administration in 24.45 mg. kg- 1 group （P 〈 0.05 ）, and at 9 days in 81.30 μg·kg-1 group （P 〈 0.05 ）. Conclu- sion： Long-term use of compound reserpine at 81.30 and 24.45 μg·kg-I can cause disturbance of intestinal microflo- ra in mice. Therefore, clinical use of the drug should pay attention to avoid the drug-related adverse reactions.